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Product Info Summary
| SKU: | A00146-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Angiogenin ANG Antibody
SKU/Catalog Number
A00146-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Angiogenin ANG Antibody (Catalog # A00146-1). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ANG antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Angiogenin ANG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00146-1)
Host
Rabbit
Contents
ANG Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ANG antibody was raised against a 15 amino acid synthetic peptide near the center of human ANG. The immunogen is located within amino acids 70 - 120 of ANG.
Cross-reactivity
Two alternatively spliced transcript variants have been observed.
Reactive Species
A00146-1 is reactive to ANG in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
16.6 kDa
Background of ANG
Angiogenin (ANG or ANG I) is important for the process of neovascularization and formation of new blood vessels. ANG is similar to pancreatic ribonuclease A and functions as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. It interacts with endothelial cell-surface actin and may cause changes in the cell cytoskeleton. ANG is thought to be involved in the development of solid tumors and its antagonists are capable of inhibiting tumor growth. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). Angiogenin is a genetic link between ALS and PD.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00146-1 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ANG antibody can be used for detection of ANG by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ANG in rat liver tissue lysate with Ang antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of ANG in human liver tissue with ANG antibody at 20 μg/mL.
Specific Publications For Anti-Angiogenin ANG Antibody (A00146-1)
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