ARHGEF5 Antibodies

AND ARHGEF5 ELISA kits, ARHGEF5 Proteins

Many biological assays use antibodies to detect ARHGEF5, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with ARHGEF5 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop ARHGEF5 antibodies...

We validate the specificity of these antibodies to ARHGEF5 by testing them on tissues known to express ARHGEF5 positively and negatively. Browse below to find the ARHGEF5 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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ARHGEF5 Infographic by Boster Bio

All ARHGEF5 Products

Product

Figures

Specificity

Applications

Price

Anti-ARHGEF5 Antibody

5 Q&As

Human, Mouse

$360
Cat # A01487-1
100 µl

Anti-ARHGEF5 Antibody

Human, Mouse, Rat

$405
Cat # A01487
100ul

ARHGEF5 (NM_005435) Human Over-expression Lysate

Human

$960
Cat # LS007984
100 µg

Anti-ARHGEF5 Antibody Picoband™

Human, Mouse, Rat

ELISA, Flow Cytometry, WB

$370
Cat # A01487-2
100 µg/vial

ARHGEF5 Overview

Facts about Rho guanine nucleotide exchange factor 5.

ARHGEF5 3D structure. Source: alphafold

Rho guanine nucleotide exchange factor 5 (ARHGEF5)

Strongly activates RHOA (PubMed:15601624). Additionally strongly activates RHOB, weakly activates RHOC and RHOG and shows no impact on RHOD, RHOV, RHOQ or RAC1 (By similarity).

Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:15601624). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (PubMed:14662653).

Gene Information

Human
Gene Name:	ARHGEF5
Uniprot:	Q12774
Entrez:	7984

Family

Belongs to:

No superfamily

Alternative Names

ephexin-3; GEF5; Guanine nucleotide regulatory protein TIM; Oncogene TIM; p60 TIM; P60; Rho guanine nucleotide exchange factor (GEF) 5; rho guanine nucleotide exchange factor 5; TIM1; TIMDKFZp686N1969; Transforming immortalized mammary oncogene

Molecular Weight

Mass (kDA):
176.799 kDA

Genomic Context


Human

Location:	7q35
Sequence:	7; NC_000007.14 (144355396..144380632)

Tissue Specificity

Ubiquitously expressed with highest levels in placenta. High levels are also found in colon, kidney, trachea, prostate, liver, pancreas, pituitary gland, thyroid gland and mammary gland. In fetal tissues, expressed at high levels in kidney, lung and liver (PubMed:15601624). Expressed at low levels in lung and heart (PubMed:14662653).

Subcellular Localizations

Cytoplasm. Nucleus. Cell projection, podosome.

Diseases

Malignant Neoplasms
Tissue Adhesions
Neoplasms
Neoplasm Metastasis
Cell Transformation, Neoplastic
Ischemia
Tumor Progression
Malignant Neoplasm Of Breast
Endometrial Neoplasms
Endometrial Polyp
Breast Fibrocystic Change, Proliferative Type
Breast Diseases

Carcinoma
Epilepsy
Asthma
Mammary Neoplasms
Endometrial Carcinoma
Inflammation
Status Epilepticus

Interacting Proteins

CALCOCO2
COG6
EFHC2
GRB2
HSPBAP1

TBC1D13
TRIM27
TRIM54

Pathways

Methylation
Cell Adhesion
Chemotaxis
Cell Migration
Leukocyte Chemotaxis
Dna Methylation
Interphase
Metaphase

Localization
Rna Interference
Dendritic Cell Migration

PTMs

Phosphorylation

Methylation

Research Areas

Cancer
GPCR

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ARHGEF5

References

PMID: 8134109 by Chan A.M.-L., et al. Expression cDNA cloning of a novel oncogene with sequence similarity to regulators of small GTP-binding proteins.

PMID: 14662653 by Debily M.A., et al. Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer.