SKU PA1013-1
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications Flow Cytometry, IHC, ICC, WB

Overview

Product Name Anti-Bax Antibody
SKU/Catalog Number PA1013-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Apoptosis regulator BAX(BAX) detection. Tested with WB, IHC-F, ICC, FCM in Human;Mouse;Rat.
Cite This Product Anti-Bax Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1013-1)
Host Rabbit
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Bax(19-33aa IMKTGALLLQGFIQD), different from the related mouse and rat sequences by one amino acid.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(F) and ICC.

Images And Assay Conditions

/antibody/pa1013 1 1 WB anti bax antibody.jpg

Figure 1. Western blot analysis of Bax using anti- Bax antibody (PA1013-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 7: A549 Cell Lysate
Lane 8: JURKAT Cell Lysate
Lane 9: Human Placenta Tissue Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Bax antigen affinity purified polyclonal antibody (Catalog # PA1013-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bax at approximately 21&28KD. The expected band size for Bax is at 21KD.

/p/a/pa1013 1 bax primary antibodies fc testing 2.jpg

Figure 2. Flow Cytometry analysis of A431 cells using anti-BAX antibody (PA1013-1).
Overlay histogram showing A431 cells stained with PA1013-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAX Antibody (PA1013-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q07812
Gene Name BAX
Protein Name Apoptosis regulator BAX
Tissue Specificity Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. .
Alternative Names Apoptosis regulator BAX;Bcl-2-like protein 4;Bcl2-L-4;BAX;BCL2L4;
Subcellular Localization Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Colocalizes with 14- 3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
Molecular Weight 21184 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. .
Background Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. Additionally, this protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

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Buy primary get secondary antibody for free.

$50 fee for conjugation. Antibody size is reduced to 50ug.

Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

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Troubleshooting

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Publications

Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats
Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3-E1 cells
Fibroblast growth factor 21 protects rat cardiomyocytes from endoplasmic reticulum stress by promoting the fibroblast growth factor receptor 1-extracellular signal-regulated kinase 1/2 signaling pathway
Ailanthone induces G2/M cell cycle arrest and apoptosis of SGC-7901 human gastric cancer cells
Ginkgolic acids induce HepG2 cell death via a combination of apoptosis, autophagy and the mitochondrial pathway
Ginsenoside Rb1 inhibit apoptosis in rat model of Alzheimer?s disease induced by A?1-40
Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils
Effects of 5-hydroxy-4?-nitro-7-propionyloxy-genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells
Magnesium isoglycyrrhizinate ameliorates doxorubicin-induced acute cardiac and hepatic toxicity via anti-oxidant and anti-apoptotic mechanisms in mice
Ouabain targets the Na+/K+-ATPase ?3 isoform to inhibit cancer cell proliferation and induce apoptosis
Downregulation of BRAF-activated non-coding RNA suppresses the proliferation, migration and invasion, and induces apoptosis of hepatocellular carcinoma cells
Blockade of sonic hedgehog signaling decreases viability and induces apoptosis in retinoblastoma cells: The key role of the PI3K/Akt pathway
Emodin alleviates severe acute pancreatitis-associated acute lung injury by decreasing pre-B-cell colony-enhancing factor expression and promoting polymorphonuclear neutrophil apoptosis
Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells
RKTG overexpression inhibits proliferation and induces apoptosis of human leukemia cells via suppression of the ERK and PI3K/AKT signaling pathways
Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats
BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway
Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure
Cardioprotective effects of traditional Chinese medicine Guanmaitong on acute myocardial infarction
Vanadium toxicity in the thymic development
Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells
Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells
Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways
Valproate Attenuates Nitroglycerin-Induced Trigeminovascular Activation by Preserving Mitochondrial Function in a Rat Model of Migraine
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway
The Effects of Fasudil at Different Doses on Acute Myocardial Infarction in Rats
Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury
Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-?B Signaling Pathway
Mesenchymal stem cells promote colorectal cancer progression through AMPK/mTOR-mediated NF-?B activation
Fenofibrate, a PPAR? agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB
Effect of curcumin on Bcl-2 and Bax expression in nude mice prostate cancer.
Adipose-Derived Mesenchymal Stem Cells Transplantation Alleviates Renal Injury in Streptozotocin-Induced Diabetic Nephropathy
A preliminary study of the effect of ECRG4 overexpression on the proliferation and apoptosis of human laryngeal cancer cells and the underlying mechanisms
Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line
Morphology and Molecular Mechanisms of Hepatic Injury in Rats under Simulated Weightlessness and the Protective Effects of Resistance Training
Agmatine protects M?ller cells from high-concentration glucose-induced cell damage via N-methyl-D-aspartic acid receptor inhibition
Alpha-lipoic acid attenuates trinitrobenzene sulfonic acid-induced ulcerative colitis in mice
Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload
Apoptosis-related protein expression in rabbits with blast brain injury following early hyperbaric oxygen therapy
Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application
The Viral Oncoprotein HBx of Hepatitis B virus Promotes the Growth of Hepatocellular Carcinoma through Cooperating with the Cellular Oncoprotein RMP
The effects of escitalopram on myocardial apoptosis and the expression of Bax and Bcl-2 during myocardial ischemia/reperfusion in a model of rats with depression
Platycodin D Induces Tumor Growth Arrest by Activating FOXO3a Expression in Prostate Cancerin vitroandin vivo
Platycodin D Induces Tumor Growth Arrest by Activating FOXO3a Expression in Prostate Cancer in vitro and in vivo
Acupuncture atBaihuiandDazhuireduces brain cell apoptosis in heroin readdicts
Olive leaf extract inhibits lead poisoning-induced brain injury
8-hydroxy-2-(di-n-propylamino)tetralin intervenes with neural cell apoptosis following diffuse axonal injury
Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms
Antitumor effects of artesunate on human breast carcinoma MCF-7 cells and IGF-IR expression in nude mice xenografts
Pathway of Programmed Cell Death and Oxidative Stress Induced by ?-Hydroxybutyrate in Dairy Cow Abomasum Smooth Muscle Cells and in Mouse Gastric Smooth Muscle
Glutamate microinjection into the hypothalamic paraventricular nucleus attenuates ulcerative colitis in rats
Tetrandrine Induces Mitochondria-Mediated Apoptosis in Human Gastric Cancer BGC-823 Cells
RMP Plays Distinct Roles in the Proliferation of Hepatocellular Carcinoma Cells and Normal Hepatic Cells
Overexpression of the hydatidiform mole-related gene F10 inhibits apoptosis in A549 cells through downregulation of BCL2-associated X protein and caspase-3
Effect of the tumor suppressor gene ING4 on the proliferation of MCF-7 human breast cancer cells
Magnolol, a natural compound, induces apoptosis of SGC-7901 human gastric adenocarcinoma cells via the mitochondrial and PI3K/Akt signaling pathways
Iptakalim, an ATP-sensitive potassium channel opener, confers neuroprotection against cerebral ischemia/reperfusion injury in rats by protecting neurovascular unit cells
RPB5-mediating Protein Is Required for the Proliferation of Hepatocellular Carcinoma Cells
In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures
Effects of garlicin on apoptosis in rat model of colitis
Chang L, Guo F, Lv Y, Wang Y, Huo B, Wang L, Liu W. Mol Biol Rep. 2013 Jan;40(1):669-73. Doi: 10.1007/S11033-012-2106-X. Epub 2012 Oct 11. The Inhibitory Effects Of Endostar Combined With Chemotherapy On Human Esophageal Squamous Cell Carcinoma Xe...
Zhao X, Chen Q, Li Y, Tang H, Liu W, Yang X. Eur J Pharm Biopharm. 2015 Jun;93:27-36. Doi: 10.1016/J.Ejpb.2015.03.003. Epub 2015 Mar 11. Doxorubicin And Curcumin Co-Delivery By Lipid Nanoparticles For Enhanced Treatment Of Diethylnitrosamine-Induc...
Lin S, Lin K, Li W, Zhou X, Huang T. Endocrine. 2010 Jun;37(3):460-6. Doi: 10.1007/S12020-010-9337-1. Epub 2010 Apr 21. Maternal Diabetes Increases Apoptosis In Mice Oocytes, Not 2-Cell Embryos.
Yan X, Wu L, Li B, Meng X, Dai H, Zheng Y, Fu J. Cardiovasc Toxicol. 2015 Jul 3. [Epub Ahead Of Print] Cyanidin-3-O-Glucoside Induces Apoptosis And Inhibits Migration Of Tumor Necrosis Factor-??-Treated Rat Aortic Smooth Muscle Cells.
Du F, Ding Y, Zou J, Li Z, Tian J, She R, Wang D, Wang H, Lv D, Chang L. Plos One. 2015 May 22;10(5):E0127047. Doi: 10.1371/Journal.Pone.0127047. Ecollection 2015. Morphology And Molecular Mechanisms Of Hepatic Injury In Rats Under Simulated Weigh...
Bu Tl, Jia Yd, Lin Jx, Mi Yl, Zhang Cq. J Zhejiang Univ Sci B. 2012 Apr;13(4):318-26. Doi: 10.1631/Jzus.B1100318. Alleviative Effect Of Quercetin On Germ Cells Intoxicated By 3-Methyl-4-Nitrophenol From Diesel Exhaust Particles.
Li Tt, Zhang Jf, Fei Sj, Zhu Sp, Zhu Jz, Qiao X, Liu Zb. Acta Pharmacol Sin. 2014 Feb;35(2):185-94. Doi: 10.1038/Aps.2013.140. Epub 2013 Dec 23. Glutamate Microinjection Into The Hypothalamic Paraventricular Nucleus Attenuates Ulcerative Colitis I...
Wang Y, Wang S, Cui W, He J, Wang Z, Yang X. Neural Regen Res. 2013 Aug 5;8(22):2021-9. Doi: 10.3969/J.Issn.1673-5374.2013.22.001. Olive Leaf Extract Inhibits Lead Poisoning-Induced Brain Injury.
Wei Q, He W, Lu Y, Yao J, Cao X. Oncol Lett. 2012 Sep;4(3):438-442. Epub 2012 Jun 7. Effect Of The Tumor Suppressor Gene Ing4 On The Proliferation Of Mcf-7 Human Breast Cancer Cells.
Zhou Yx, Qiu Yq, Xu Lq, Guo J, Li Lj. Afr J Tradit Complement Altern Med. 2012 Apr 2;9(3):405-11. Ecollection 2012. Xiao-Chai-Hu Tang In Treating Model Mice With D-Galactosamine-Induced Liver Injury.
Song Y, Zhang G, Zhu X, Pang Z, Xing F, Quan S. Oncol Lett. 2012 Sep;4(3):419-422. Epub 2012 Jun 19. Overexpression Of The Hydatidiform Mole-Related Gene F10 Inhibits Apoptosis In A549 Cells Through Downregulation Of Bcl2-Associated X Protein And ...
Sun Y, Xu Hj, Zhao Yx, Wang Lz, Sun Lr, Wang Z, Sun Xf. Evid Based Complement Alternat Med. 2013;2013:690164. Doi: 10.1155/2013/690164. Epub 2013 Mar 19. Crocin Exhibits Antitumor Effects On Human Leukemia Hl-60 Cells In Vitro And In Vivo.
Shuai H, Zhang J, Zhang J, Xie J, Zhang M, Yu Y, Zhang L. Endocr Res. 2011;36(1):25-34. Doi: 10.3109/07435800.2010.534753. Erythropoietin Protects Pancreatic ??-Cell Line Nit-1 Cells Against Cytokine-Induced Apoptosis Via Phosphatidylinositol 3-Kin...
Chen J, Huang Rb. Neurosciences (Riyadh). 2009 Oct;14(4):343-8. Protective Effect Of Yulangsan Polysaccharide On Focal Cerebral Ischemia/Reperfusion Injury In Rats And Its Underlying Mechanism.
Liu W, Zheng Q, Wang Y, Han X, Yuan L, Zhao M. Neurochem Res. 2015 Jan;40(1):70-80. Doi: 10.1007/S11064-014-1467-Z. Epub 2014 Nov 5. Transplantation Of Olfactory Ensheathing Cells Attenuates Acute Carbon Monoxide Poisoning-Induced Brain Damages In...
Yuan F, Dong P, Wang X, Fu X, Dai M, Zhang W. Exp Toxicol Pathol. 2013 Nov;65(7-8):1011-8. Doi: 10.1016/J.Etp.2013.02.004. Epub 2013 Mar 19. Toxicological Effects Of Cigarette Smoke On Ana-1 Macrophages In Vitro.
Luo Q, Li Z, Yan J, Zhu F, Xu Rj, Cai Yz. J Med Food. 2009 Aug;12(4):695-703. Doi: 10.1089/Jmf.2008.1232. Lycium Barbarum Polysaccharides Induce Apoptosis In Human Prostate Cancer Cells And Inhibits Prostate Cancer Growth In A Xenograft Mouse Mode...
Wu Cy, Tang Zh, Jiang L, Li Xf, Jiang Zs, Liu Ls. Mol Cell Biochem. 2012 Jan;359(1-2):347-58. Doi: 10.1007/S11010-011-1028-6. Epub 2011 Aug 17. Pcsk9 Sirna Inhibits Huvec Apoptosis Induced By Ox-Ldl Via Bcl/Bax-Caspase9-Caspase3 Pathway.
Rasul A, Yu B, Zhong L, Khan M, Yang H, Ma T. Oncol Rep. 2012 May;27(5):1481-7. Doi: 10.3892/Or.2012.1694. Epub 2012 Feb 21. Cytotoxic Effect Of Evodiamine In Sgc-7901 Human Gastric Adenocarcinoma Cells Via Simultaneous Induction Of Ap...
Li Y, Gu Wj, Liu Hl. Dig Dis Sci. 2012 Aug;57(8):2072-8. Doi: 10.1007/S10620-012-2132-5. Epub 2012 May 10. Induction Of Pancreatic Cancer Cell Apoptosis And Enhancement Of Gemcitabine Sensitivity By Rap80 Sirna.
Wang Y, Liu X, Zhang D, Chen J, Liu S, Berk M. Bmc Med. 2013 Feb 8;11:32. Doi: 10.1186/1741-7015-11-32. The Effects Of Apoptosis Vulnerability Markers On The Myocardium In Depression After Myocardial Infarction.
Gu Wj, Liu Hl. Anticancer Drugs. 2013 Jul;24(6):566-76. Doi: 10.1097/Cad.0B013E3283608Bc5. Induction Of Pancreatic Cancer Cell Apoptosis, Invasion, Migration, And Enhancement Of Chemotherapy Sensitivity Of Gemcitabine, 5-Fu, And Oxaliplatin By Hnr...
Wu K, Liu Y, Lv Y, Cui L, Li W, Chen J, Liang Nc, Li L. Oncol Rep. 2013 Jun;29(6):2101-8. Doi: 10.3892/Or.2013.2375. Epub 2013 Apr 3. Ent-11??-Hydroxy-15-Oxo-Kaur-16-En-19-Oic-Acid Induces Apoptosis And Cell Cycle Arrest In Cne-2Z Nasopharyngeal Ca...
Qin R, Shen H, Cao Y, Fang Y, Li H, Chen Q, Xu W. Plos One. 2013 Oct 1;8(10):E76486. Doi: 10.1371/Journal.Pone.0076486. Ecollection 2013. Tetrandrine Induces Mitochondria-Mediated Apoptosis In Human Gastric Cancer Bgc-823 Cells.
Wei T, Tian W, Liu F, Xie G. Biochem Biophys Res Commun. 2014 May 16;447(4):666-71. Doi: 10.1016/J.Bbrc.2014.04.074. Epub 2014 Apr 19. Protective Effects Of Exogenous ??-Hydroxybutyrate On Paraquat Toxicity In Rat Kidney.
Wang Y, Zhang H, Chai F, Liu X, Berk M,,,,. Bmc Psychiatry. 2014 Dec 4;14:349. Doi: 10.1186/S12888-014-0349-X. The Effects Of Escitalopram On Myocardial Apoptosis And The Expression Of Bax And Bcl-2 During Myocardial Ischemia/Reperfusion In A Mode...
Wu C, Dong S, Li Y. Int J Mol Med. 2015 Apr;35(4):893-900. Doi: 10.3892/Ijmm.2015.2105. Epub 2015 Feb 18. Effects Of Mirna-455 On Cardiac Hypertrophy Induced By Pressure Overload.
Han N, Yu L, Song Z, Luo L, Wu Y. Mol Med Rep. 2015 Jul;12(1):1098-106. Doi: 10.3892/Mmr.2015.3540. Epub 2015 Mar 24. Agmatine Protects M??ller Cells From High-Concentration Glucose-Induced Cell Damage Via N-Methyl-D-Aspartic Acid Receptor Inhibition.
Sun J, Zhang H, Guan L, Zhou H, Sun M. Int J Clin Exp Med. 2015 Jan 15;8(1):358-67. Ecollection 2015. Alpha-Lipoic Acid Attenuates Trinitrobenzene Sulfonic Acid-Induced Ulcerative Colitis In Mice.
Rasul A, Ding C, Li X, Khan M, Yi F, Ali M, Ma T. Apoptosis. 2012 Oct;17(10):1104-19. Doi: 10.1007/S10495-012-0742-1. Dracorhodin Perchlorate Inhibits Pi3K/Akt And Nf-??b Activation, Up-Regulates The Expression Of P53, And Enhances Apoptosis.
Yin W, Li B, Li X, Yu F, Cai Q, Zhang Z, Wang J, Zhang J, Zhou R, Cheng M, Gao H. J Cardiovasc Pharmacol. 2015 Jan;65(1):13-21. Doi: 10.1097/Fjc.0000000000000157. Critical Role Of Prohibitin In Endothelial Cell Apoptosis Caused By Glycated Low-Den...
Zhou Y, Tan X, Kuang W, Liu L, Wan L. Transl Res. 2012 Jun;159(6):464-72. Doi: 10.1016/J.Trsl.2011.09.007. Epub 2011 Oct 24. Erythromycin Ameliorates Cigarette-Smoke-Induced Emphysema And Inflammation In Rats.
Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.
Ban Jb, Fan Xw, Huang Q, Li Bf, Chen C, Zhang Hc, Xu Sq. Plos One. 2014 May 16;9(5):E97607. Doi: 10.1371/Journal.Pone.0097607. Ecollection 2014. Mono-(2-Ethylhexyl) Phthalate Induces Injury In Human Umbilical Vein Endothelial Cells.
Wang Jm, Xiao Bl, Zheng Jw, Chen Hb, Zou Sq. World J Gastroenterol. 2007 Jun 21;13(23):3171-5. Effect Of Targeted Magnetic Nanoparticles Containing 5-Fu On Expression Of Bcl-2, Bax And Caspase 3 In Nude Mice With Transplanted Human Liver Cancer.
Meng Qt, Xia Zy, Luo T, Cao C, Zhao B, Wu Y, Wu X, Chen X. Saudi Med J. 2012 Feb;33(2):139-45. Ligustrazine Attenuates Acute Lung Injury Induced By Blunt Chest Trauma.
Zhou Y, Li W, Xu Q, Huang Y. J Exp Clin Cancer Res. 2010 Oct 4;29:131. Doi: 10.1186/1756-9966-29-131. Elevated Expression Of Dickkopf-1 Increases The Sensitivity Of Human Glioma Cell Line Shg44 To Bcnu.
Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T, Yang H. Int J Oncol. 2012 Apr;40(4):1153-61. Doi: 10.3892/Ijo.2011.1277. Epub 2011 Nov 30. Magnolol, A Natural Compound, Induces Apoptosis Of Sgc-7901 Human Gastric Adenocarcinoma Cells Vi...
Peng X, Chen K, Chen J, Fang J, Cui H, Zuo Z, Deng J, Chen Z, Geng Y, Lai W. Environ Toxicol. 2015 Mar 4. Doi: 10.1002/Tox.22120. [Epub Ahead Of Print] Aflatoxin B1 Affects Apoptosis And Expression Of Bax, Bcl-2, And Caspase-3 In Thymus And Bursa ...
Chen J, Li X, Qiu J, You H, Zhang Q, Dong G, Zuo Y. Cell Biochem Biophys. 2013;67(3):1015-9. Doi: 10.1007/S12013-013-9597-5. Kinetics Of Apoptosis And Expression Of Apoptosis-Related Proteins In Rat Ca3 Hippocampus Cells After Experimental Diffuse...
Liu Y, Zhou Y, Wang Y, Geng D, Liu J. Obes Surg. 2011 Sep;21(9):1424-31. Doi: 10.1007/S11695-011-0388-Z. Roux-En-Y Gastric Bypass-Induced Improvement Of Glucose Tolerance And Insulin Resistance In Type 2 Diabetic Rats Are Mediated By Glucagon-Like...
Yang Y, Li Zh, Liu H, Shi Wd, Zhang J. Chin J Integr Med. 2015 Jun;21(6):423-30. Doi: 10.1007/S11655-014-1752-3. Epub 2014 May 10. Inhibitory Effect Of Tetramethylpyrazine Preconditioning On Overload Training-Induced Myocardial Apoptosis In Rats.
Song Gh, Wang Rl, Chen Zy, Zhang B, Wang Hl, Liu Ml, Gao Jp, Yan Xy. J Physiol Biochem. 2014 Sep;70(3):761-8. Doi: 10.1007/S13105-014-0344-1. Epub 2014 Jul 30. Toxic Effects Of Sodium Fluoride On Cell Proliferation And Apoptosis Of Leydig Cells Fr...
Chen K, Shu G, Peng X, Fang J, Cui H, Chen J, Wang F, Chen Z, Zuo Z, Deng J, Geng Y, Lai W. Food Chem Toxicol. 2013 Sep;59:446-54. Doi: 10.1016/J.Fct.2013.06.032. Epub 2013 Jun 28. Protective Role Of Sodium Selenite On Histopathological Lesions, D...
Mao Z, Song Z, Li G, Lv W, Zhao X, Li B, Feng X, Chen Y. Neural Regen Res. 2013 Jan 15;8(2):133-42. Doi: 10.3969/J.Issn.1673-5374.2013.02.005. 8-Hydroxy-2-(Di-N-Propylamino)Tetralin Intervenes With Neural Cell Apoptosis Following Diffuse Axonal In...
Yan Y, Liang B, Zhang J, Liu Y, Bu X. Mol Med Rep. 2015 Jan;11(1):317-26. Doi: 10.3892/Mmr.2014.2657. Epub 2014 Oct 15. Apoptotic Induction Of Lung Adenocarcinoma A549 Cells Infected By Recombinant Rvg Newcastle Disease Virus (Rl-Rvg) In Vitro.
Li X, Wang L, Wang Z. Phys Med. 2015 Jun;31(4):352-9. Doi: 10.1016/J.Ejmp.2015.02.004. Epub 2015 Feb 19. Radioprotective Activity Of Neutral Polysaccharides Isolated From The Fruiting Bodies Of Hohenbuehelia Serotina.
Wei T, Tian W, Yan H, Shao G, Xie G. Cell Mol Neurobiol. 2014 Nov;34(8):1165-73. Doi: 10.1007/S10571-014-0091-4. Epub 2014 Aug 22. Protective Effects Of Phillyrin On H2O 2-Induced Oxidative Stress And Apoptosis In Pc12 Cells.
Ding H, Wang H, Zhao Y, Sun D, Zhai X. Cell Mol Neurobiol. 2015 Jul;35(5):623-32. Doi: 10.1007/S10571-015-0156-Z. Epub 2015 Jan 18. Protective Effects Of Baicalin On A??????????????-Induced Learning And Memory Deficit, Oxidative Stress, And Apoptosis In Rat.
Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W. Neural Regen Res. 2012 Jun 15;7(17):1318-24. Doi: 10.3969/J.Issn.1673-5374.2012.17.006. Apoptosis-Related Protein Expression In Rabbits With Blast Brain Injury Following Early Hyperbaric ...
Khan M, Li T, Ahmad Khan Mk, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T. Biomed Res Int. 2013;2013:719858. Doi: 10.1155/2013/719858. Epub 2012 Dec 27. Alantolactone Induces Apoptosis In Hepg2 Cells Through Gsh Depletion, Inhibition Of Stat3 Activation...
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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.