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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||Flow Cytometry, WB|
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Anti-Apoptosis regulator BAX Bax Antibody
Boster Bio Anti-Apoptosis regulator BAX Bax Antibody catalog # PA1013-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apoptosis regulator BAX Bax Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1013-1)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of human Bax (19-33aa IMKTGALLLQGFIQD), different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
PA1013-1 is reactive to BAX in Human, Mouse, Rat
PA1013-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Background of BAX
Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. Additionally, this protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Bax using anti-Bax antibody (PA1013-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 7: A549 Cell Lysate
Lane 8: JURKAT Cell Lysate
Lane 9: Human Placenta Tissue Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bax antigen affinity purified polyclonal antibody (Catalog # PA1013-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bax at approximately 21&28KD. The expected band size for Bax is at 21KD.
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Figure 2. Flow Cytometry analysis of A431 cells using anti-BAX antibody (PA1013-1).
Overlay histogram showing A431 cells stained with PA1013-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAX Antibody (PA1013-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Gene/Protein Information For BAX (Source: Uniprot.org, NCBI)
Apoptosis regulator BAX
apoptosis regulator BAX; Bax; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4 BAX BCL2L4 BCL2 associated X, apoptosis regulator apoptosis regulator BAX|BCL2 associated X protein|BCL2-associated X protein omega|Baxdelta2G9|Baxdelta2G9omega|Baxdelta2omega|bcl-2-like protein 4|bcl2-L-4*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on BAX, check out the BAX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
PA1013-1 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng K,Yuan G,Bao H,Wang L,Ma R,Yu B,Zhao S.Interaction of HCCR-1 and Bax in breast cancer.J BUON.2019 May-Jun;24(3):1027-1037.PMID:31424657.
PA1013-1 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:NA
Su-Su Tang,Yi Ren,Xiao-Qian Ren,Jing-Ran Cao,Hao Hong,Hui Ji,Qing-Hua Hu,ERα and/or ERβ activation ameliorates cognitive impairment, neurogenesis and apoptosis in type 2 diabetes mellitus mice,Experimental Neurology,Volume 311,2019,Pages 33-43,ISSN 0014-4886,https://doi.org/10.1016/j.expneurol.2018.09.002.
PA1013-1 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:1:500
Ren Q,Hu Z,Jiang Y,Tan X,Botchway BOA,Amin N,Lin G,Geng Y,Fang M.SIRT1 Protects Against Apoptosis by Promoting Autophagy in the Oxygen Glucose Deprivation/Reperfusion-Induced Injury.Front Neurol.2019 Dec 5;10:1289.doi:10.3389/fneur.2019.01289.PMID:31920915;PMCID:PMC6915092.
PA1013-1 usage in article: APP:WB, SAMPLE: PC12 CELL, DILUTION:1:400
Zhang X,Guo S,Huang X,Li B,Dai H,Wang C.A novel macrolide derivative ameliorates smoke-induced inflammation and emphysema by inhibiting NF-κB activation.Am J Transl Res.2021 Mar 15;13(3):1209-1220.PMID:33841650;PMCID:PMC8014339.
PA1013-1 usage in article: APP:IHC, SAMPLE:TRACHEAL TISSUE, DILUTION:1:50
Song P, Yan B, Lei F, Qiu Z, Zhang C, Wu Y, Chen S, Yang X, Shen D, Ma P. Continuous artificial light at night exacerbates diisononyl phthalate-induced learning and memory impairment in mice: Toxicological evidence. Food Chem Toxicol. 2021 Mar 9;151: 112102. doi: 10.1016/j.fct.2021.112102. Epub ahead of print. PMID: 33711377.
PA1013-1 usage in article: APP:IHC, SAMPLE: HIPPOCAMPUL TISSUE, DILUTION:NA
Junyi Tang,Kehua Fang,Chang Li,Xiaotian Chang,"ARHGEF10L Promotes Cervical Tumorigenesis via RhoA-Mediated Signaling",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 6683264,11 pages,2021.https://doi.org/10.1155/2021/6683264
PA1013-1 usage in article: APP:WB, SAMPLE:HELA CELL, DILUTION:NA
Zhang Z, Liu W, Shen M, Ma X, Li R, Jin X, Bai H, Gao L. Protective Effect of GM1 Attenuates Hippocampus and Cortex Apoptosis After Ketamine Exposure in Neonatal Rat via PI3K/AKT/GSK3β Pathway. Mol Neurobiol. 2021 Mar 17. doi: 10.1007/s12035-021-02346-5.Epub ahead of print.PMID: 33733293.
PA1013-1 usage in article: APP:IHC, SAMPLE:TUMOR TISSUE, DILUTION:NA
Liang,T.,Wei,C.,Lu,S.,Qin,M.,Qin,G.,Zhang,Y. ... Yang,Y. (2021).Ginaton injection alleviates cisplatin‑induced renal interstitial fibrosis in rats via inhibition of apoptosis through regulation of the p38MAPK/TGF‑β1 and p38MAPK/HIF‑1α pathways.Biomedical
PA1013-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:200
Xu Y,Chen W,Chen Z,Huang M,Yang F,Zhang Y.Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway.Drug Des Devel Ther.2021 Feb 22;15:753-767.doi:10.2147/DDDT.S280217.PMID:33654381;
PA1013-1 usage in article: APP:WB, SAMPLE:PC12 CELL, DILUTION:1:500
Zhou L,Liu Y,Wang Z,Liu D,Xie B,Zhang Y,Yuan M,Tse G,Li G,Xu G,Liu T.Activation of NADPH oxidase mediates mitochondrial oxidative stress and atrial remodeling in diabetic rabbits.Life Sci.2021 Feb 15:119240.doi:10.1016/j.lfs.2021.119240.Epub ahead of prin
PA1013-1 usage in article: APP:WB, SAMPLE:ATRIAL TISSUE, DILUTION:1:300
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