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- Table of Contents
CACNA1S · Western blot design guide
CACNA1S Western Blot Planning Guide
Plan a CACNA1S Western blot around the catalog-observed 212.4 kDa band, image-backed A03006-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
CACNA1S Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 212.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 4-12% gradient | |
| Positive control | Skeletal muscle | |
| Negative control | Adipose tissue |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 212.4 kDa |
| ⓘ | Localization | Cell membrane, sarcolemma, T-tubule |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated CACNA1S Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Skeletal muscle |
| Gel % | 4-12% gradient |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A03006-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected CACNA1S Western Blot Band Size?
Use the product-observed 212.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 212.4 kDa | Matches the authoritative product WB observation. |
| 212.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected CACNA1S appearancePlan around 212.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 212.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 212.4 kDa from UniProt Q13698; retain as context. |
| Gel selection | 4-12% gradient; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A03006-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 212.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing CACNA1S band
A03006-1 · CACNA1S
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CACNA1S Western blot
🧪Use Skeletal muscle as the first positive-control candidate and Adipose tissue as the HPA Not detected negative candidate.
Positive control: Skeletal muscle (High)
Negative control: Adipose tissue (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for CACNA1S
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Section 3
Advanced CACNA1S Western Blot Tips
Deeper troubleshooting and optimisation questions for CACNA1S, answered from its protein features.
Which band should guide the blot?
Use 212.4 kDa, the observation attached to the authoritative A03006-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 212.4 kDa expectation.
Which positive control should I start with?
Start with Skeletal muscle, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adipose tissue as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 4-12% gradient consistently across the quick facts, protocol table, and poster.
What transfer method to use for CACNA1S Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A03006-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
CACNA1S Western Blot Reagents
Human/Mouse/Rat-reactive CACNA1S Western blot reagents with authoritative product imagery.
Real WB data
Anti-CACNA1S Antibody Picoband®
Cat # A03006-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q13698
- Human Protein Atlas — CACNA1S
- A03006-1 product record
- PMC6834399 — Increased calcium channel in the lamina propria of aging rat (Aging, 2019)
- PMC4854922 — Calpain inhibition rescues troponin T3 fragmentation, increases Cav1.1, and enhances skeletal muscle force in aging sedentary mice (Aging cell, 2016)
- PMC8675865 — Screening and Identification of Lujo Virus Entry Inhibitors From an Food and Drug Administration-Approved Drugs Library (Frontiers in microbiology, 2021)