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- Table of Contents
CDC27 · Western blot design guide
CDC27 Western Blot Planning Guide
Plan a CDC27 Western blot around the catalog-observed 91.9 kDa band, image-backed A03905-3 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
CDC27 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 91.9 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Cervix | |
| Negative control | Heart muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 91.9 kDa |
| ⓘ | Localization | Nucleus / Cytoplasm, cytoskeleton, spindle |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated CDC27 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Bone marrow |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A03905-3 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected CDC27 Western Blot Band Size?
Use the product-observed 91.9 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 91.9 kDa | Matches the authoritative product WB observation. |
| 91.9 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected CDC27 appearancePlan around 91.9 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 91.9 kDa; use this as the primary experimental expectation. |
| Calculated mass | 91.9 kDa from UniProt P30260; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A03905-3. |
| Situation | Likely cause | Next action |
|---|---|---|
| 91.9 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing CDC27 band
A03905-3 · CDC27
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CDC27 Western blot
🧪Use Cervix as the first positive-control candidate and Heart muscle as the HPA Not detected negative candidate.
Positive control: Cervix (High)
Negative control: Heart muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for CDC27
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cervix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Colon | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bone marrow | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Heart muscle | Reported tissue cells | Not detected | Protein (HPA) | HPA → |
Section 3
Advanced CDC27 Western Blot Tips
Deeper troubleshooting and optimisation questions for CDC27, answered from its protein features.
Which band should guide the blot?
Use 91.9 kDa, the observation attached to the authoritative A03905-3 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 91.9 kDa expectation.
Which positive control should I start with?
Start with Cervix, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Heart muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for CDC27 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A03905-3 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
CDC27 Western Blot Reagents
Human/Mouse/Rat-reactive CDC27 Western blot reagents with authoritative product imagery.
Real WB data
Anti-CDC27 Antibody Picoband®
Cat # A03905-3
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P30260
- Human Protein Atlas — CDC27
- A03905-3 product record
- PMC4955705 — PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27 (Molecular cancer research : MCR, 2016)
- PMC2788612 — PP1-mediated dephosphorylation of phosphoproteins at mitotic exit is controlled by inhibitor-1 and PP1 phosphorylation (Nature cell biology, 2009)
- PMC3296673 — Anaphase-promoting complex/cyclosome protein Cdc27 is a target for curcumin-induced cell cycle arrest and apoptosis (BMC cancer, 2012)
