CDK5RAP2 · Western blot design guide

CDK5RAP2 Western Blot Planning Guide

Plan a CDK5RAP2 Western blot around the catalog-observed 215 kDa band, image-backed A03102-2 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for CDK5RAP2 (CDK5RAP2): expected band 215 kDa, antibody A03102-2, and PMC-cited SDS-PAGE protocol steps

Band Protocols Controls Antibodies

CDK5RAP2 Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	215 kDa
	Observed band	Not reported — verify product WB image
	Gel	4-12% gradient
	Positive control ⓘ	Cervix
	Negative control ⓘ	Target knockdown/knockout

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	215 kDa
ⓘ	Localization	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome / Golgi apparatus
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human

Sample controls for CDK5RAP2 Western blot

🧪Use Cervix as the first positive-control candidate; no defensible HPA Not detected tissue was available, so use a target knockdown/knockout negative control.

Positive control: Cervix (High)

Negative control: Target knockdown/knockout

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for CDK5RAP2

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Cervix	Reported tissue cells	High	Protein (HPA)	HPA →
Breast	Reported tissue cells	High	Protein (HPA)	HPA →
Adrenal gland	Reported tissue cells	High	Protein (HPA)	HPA →
Appendix	Reported tissue cells	High	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source

No HPA Not detected tissue is available; use a target knockdown/knockout negative control.

Section 1

What Is the Expected CDK5RAP2 Western Blot Band Size?

Use the product-observed 215 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
215 kDa	Matches the authoritative product WB observation.
215 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected CDK5RAP2 appearancePlan around 215 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	215 kDa; use this as the primary experimental expectation.
Calculated mass	215 kDa from UniProt Q96SN8; retain as context.
Gel selection	4-12% gradient; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with A03102-2.

Why is my band missing or off?
Situation	Likely cause	Next action
215 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt Q96SN8 Human Protein Atlas product datasheet (A03102-2)

Experiment evidence

Western blot images showing CDK5RAP2 band

A03102-2 · CDK5RAP2 — observed 215 kDa WB validation

A03102-2 · CDK5RAP2Observed 215 kDa · product-specific WB image

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Section 2

Real Curated CDK5RAP2 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Adrenal gland
Gel %	4-12% gradient
Load	20-30 µg total protein per lane
Transfer	Wet/tank, extended transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	A03102-2 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 4

Advanced CDK5RAP2 Western Blot Tips

Deeper troubleshooting and optimisation questions for CDK5RAP2, answered from its protein features.

Which band should guide the blot?

Use 215 kDa, the observation attached to the authoritative A03102-2 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 215 kDa expectation.

Which positive control should I start with?

Start with Cervix, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use a target knockdown/knockout control when no HPA Not detected tissue is available.

Which gel should I use?

Use 4-12% gradient consistently across the quick facts, protocol table, and poster.

What transfer method to use for CDK5RAP2 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should A03102-2 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC8688469, PMC9289712, PMC11269669.

Boster reagents

CDK5RAP2 Western Blot Reagents

Human-reactive CDK5RAP2 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for CDK5RAP2 using A03102-2; observed band 215 kDa

Anti-CDK5RAP2 Antibody Picoband®

Cat # A03102-2

Reactivity: Human

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.