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- Table of Contents
COL4A2 · Western blot design guide
COL4A2 Western Blot Planning Guide
Plan a COL4A2 Western blot around the catalog-observed 167.6 kDa band, image-backed PA1521 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
COL4A2 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 167.6 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Placenta | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 167.6 kDa |
| ⓘ | Localization | Secreted, extracellular space, extracellular matrix, basement membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Sample controls for COL4A2 Western blot
🧪Use Placenta as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Placenta (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for COL4A2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Placenta | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 1
What Is the Expected COL4A2 Western Blot Band Size?
Use the product-observed 167.6 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 167.6 kDa | Matches the authoritative product WB observation. |
| 167.6 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected COL4A2 appearancePlan around 167.6 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 167.6 kDa; use this as the primary experimental expectation. |
| Calculated mass | 167.6 kDa from UniProt P08572; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PA1521. |
| Situation | Likely cause | Next action |
|---|---|---|
| 167.6 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing COL4A2 band
PA1521 · COL4A2Observed 167.6 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Section 2
Real Curated COL4A2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Placenta |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PA1521 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 4
Advanced COL4A2 Western Blot Tips
Deeper troubleshooting and optimisation questions for COL4A2, answered from its protein features.
Which band should guide the blot?
Use 167.6 kDa, the observation attached to the authoritative PA1521 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 167.6 kDa expectation.
Which positive control should I start with?
Start with Placenta, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for COL4A2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PA1521 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC11361908, PMC12705385, PMC13005436.
Boster reagents
COL4A2 Western Blot Reagents
Human-reactive COL4A2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-Canstatin Antibody Picoband®
Cat # PA1521
Reactivity: Human
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P08572
- Human Protein Atlas — COL4A2
- PA1521 product record
- PMC11361908 — COL4A2 enhances thyroid cancer cell proliferation through the AKT pathway.
- PMC12705385 — Integration of single-cell sequencing and machine learning identifies key macrophage-associated genetic signatures in lumbar disc degeneration.
- PMC13005436 — Metamorphosing macrophages promote placental fibrosis by upregulating the transcription factor STAT5A.