DPH5 Antibodies

AND DPH5 ELISA kits, DPH5 Proteins

Many biological assays use antibodies to detect DPH5, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DPH5 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DPH5 antibodies...

We validate the specificity of these antibodies to DPH5 by testing them on tissues known to express DPH5 positively and negatively. Browse below to find the DPH5 antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for DPH5? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the DPH5 Western Blot Guide

DPH5 Infographic by Boster Bio

All DPH5 Products

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Product

Figures

Specificity

Applications

Price

Anti-DPH5 Antibody Picoband®

Western blot analysis of DPH5 using anti-DPH5 antibody (A11989-1)

IF analysis of DPH5 using anti-DPH5 antibody (A11989-1)

Flow Cytometry analysis of A549 cells using anti-DPH5 antibody (A11989-1)

Human

ELISA, Flow Cytometry, IF, ICC, WB

$370
Cat # A11989-1
100 μg/vial

DPH5 (NM_015958) Human Over-expression Lysate

DPH5 Human Over-expression Lysates NM_001077395

Human

$628
Cat # LS051611
20 µg, 100 µg

DPH5 Overview

Facts about Diphthine methyl ester synthase.

DPH5 3D structure. Source: alphafold

Diphthine methyl ester synthase (DPH5)

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the altered target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.

Gene Information

Human
Gene Name:	DPH5
Uniprot:	Q9H2P9
Entrez:	51611

Family

Belongs to:

diphthine synthase family

Alternative Names

CGI-30; Diphthamide biosynthesis methyltransferase; diphthine synthase; DPH5 homolog (S. cerevisiae); EC 2.1.1.98; HSPC143; MGC61450; NPD015; protein x 0011

Molecular Weight

Mass (kDA):
31.651 kDA

Genomic Context


Human

Location:	1p21.2
Sequence:	1; NC_000001.11 (100975218..101025806, complement)

Diseases

Diphtheria
Neoplasms
Polydactyly
Radial Polydactyly
Poisoning

Pathways

Translation
Methylation
Trna Modification
Localization

PTMs

Amidation

Methylation

Ribosylation

Research Areas

Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DPH5

References

PMID: 23486472 by Wei H., et al. A modified form of diphthamide causes immunotoxin resistance in a lymphoma cell line with a deletion of the WDR85 gene.