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- Table of Contents
EOMES · Western blot design guide
EOMES Western Blot Planning Guide
Plan an EOMES Western blot around the catalog-observed 72.7 kDa band, image-backed A00992-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
EOMES Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 72.7 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Parathyroid gland | |
| Negative control | Oral mucosa |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 72.7 kDa |
| ⓘ | Localization | Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated EOMES Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Parathyroid gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A00992-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected EOMES Western Blot Band Size?
Use the product-observed 72.7 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 72.7 kDa | Matches the authoritative product WB observation. |
| 72.7 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected EOMES appearancePlan around 72.7 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 72.7 kDa; use this as the primary experimental expectation. |
| Calculated mass | 72.7 kDa from UniProt O95936; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A00992-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 72.7 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing EOMES band
A00992-1 · EOMES
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for EOMES Western blot
🧪Use Parathyroid gland as the first positive-control candidate and Oral mucosa as the HPA Not detected negative candidate.
Positive control: Parathyroid gland (Medium)
Negative control: Oral mucosa (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for EOMES
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Parathyroid gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Tonsil | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 3
Advanced EOMES Western Blot Tips
Deeper troubleshooting and optimisation questions for EOMES, answered from its protein features.
Which band should guide the blot?
Use 72.7 kDa, the observation attached to the authoritative A00992-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 72.7 kDa expectation.
Which positive control should I start with?
Start with Parathyroid gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Oral mucosa as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for EOMES Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A00992-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
EOMES Western Blot Reagents
Human/Mouse/Rat-reactive EOMES Western blot reagents with authoritative product imagery.
Real WB data
Anti-TBR2/Eomes Antibody Picoband®
Cat # A00992-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt O95936
- Human Protein Atlas — EOMES
- A00992-1 product record
- PMC3140599 — Eomesodermin, HAND1, and CSH1 proteins are induced by cellular stress in a stress-activated protein kinase-dependent manner (Molecular reproduction and development, 2011)
- PMC10847424 — Heat shock protein gp96 drives natural killer cell maturation and anti-tumor immunity by counteracting Trim28 to stabilize Eomes (Nature communications, 2024)
- PMC5035225 — Reciprocal regulation of BMF and BIRC5 (Survivin) linked to Eomes overexpression in colorectal cancer (Cancer letters, 2016)