FKHR Colorimetric Cell-Based ELISA

The FKHR Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FKHR protein expression profile in cells. The kit can be used for measuring the relative amounts of FKHR in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FKHR.

Product Info Summary

SKU: EKC1930
Size: 1 kit, containing 1 plates and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

Product Name

FKHR Colorimetric Cell-Based ELISA

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SKU/Catalog Number

EKC1930

Size

1 kit, containing 1 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The FKHR Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FKHR protein expression profile in cells. The kit can be used for measuring the relative amounts of FKHR in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FKHR.

Storage & Handling

4°C/6 Months

Cite This Product

FKHR Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1930)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1930 is reactive to FOXO1 in Human, Mouse samples

Application Guarantee

EKC1930 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-FOXO1 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For FOXO1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q12778

Gene ID

2308

Gene Name

FOXO1

Full Name

Forkhead box protein O1

Weight

69.662kDa

Alternative Names

FKH1; FKHR; FKHRforkhead box protein O1; forkhead box O1; Forkhead box protein O1A; forkhead homolog in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma; FoxO1; FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma FOXO1 FKH1, FKHRA, FOXO1 forkhead box O1 forkhead box protein O1|forkhead box protein O1A|forkhead, Drosophila, homolog of, in rhabdomyosarcoma

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FOXO1, check out the FOXO1 Infographic

FOXO1 infographic

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In this infographic you will see the following information for FOXO1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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