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Product Info Summary
| SKU: | A00073 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Forkhead box protein O1 FOXO1 Antibody
SKU/Catalog Number
A00073
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Forkhead box protein O1 FOXO1 Antibody (Catalog # A00073). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
FOXO1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Forkhead box protein O1 FOXO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00073)
Host
Rabbit
Contents
FOXO1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FOXO1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human FOXO1. The immunogen is located within the first 50 amino acids of FOXO1.
Reactive Species
A00073 is reactive to FOXO1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
69.7 kDa
Background of FOXO1
FOXO1, also known as FKHR or ForkHead in Rhabdomyosarcoma, is a 70 kDa protein which is a ubiquitously expressed member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In insulin-responsive tissues, stress or nutrient abundance triggers phosphorylation by PKB/AKT, blocking nuclear translocation and activity. Genetic mutations involving FOXO1A are a cause of alveolar rhabdomyosarcoma (RMS2). Recent studies link the anti-tumor activity of FOXO1 and the process of autophagy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00073 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FOXO1 antibody can be used for detection of FOXO1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FOXO1 in Hela cell lysate with FOXO1 antibody at 1 μg/mL.
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Immunocytochemistry of FOXO1 in HeLa cells with FOXO1 antibody at 4 μg/mL.
Specific Publications For Anti-Forkhead box protein O1 FOXO1 Antibody (A00073)
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