GSK3B Antibodies

Glycogen synthase kinase-3 beta (GSK3B)

Constitutively active protein kinase that functions as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates.

In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 action and hence glycogen synthesis. May also mediate the progression of insulin resistance by regulating activation of transcription factors.

Gene Information

Human
Gene Name:	GSK3B
Uniprot:	P49841
Entrez:	2932

Family

Belongs to:
protein kinase superfamily

Alternative Names

EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform

Molecular Weight

Mass (kDA):
46.744 kDA

Genomic Context

Human
Location:	3q13.33
Sequence:	3; NC_000003.12 (119821321..120095823, complement)

Tissue Specificity

Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.

Subcellular Localizations

Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane.

Diseases

• Malignant Neoplasms
• Neoplasms
• Depressive Disorder
• Alzheimer's Disease
• Bipolar Disorder
• Insulin Resistance
• Major Depressive Disorder
• Schizophrenia
• Diabetes Mellitus
• Malignant Paraganglionic Neoplasm
• Hypertrophy
• Parkinson Disease
• Dementia

• Impaired Cognition
• Neurodegenerative Disorders
• Adenocarcinoma
• Polycystic Ovary Syndrome
• Neurofibrillary Tangles
• Diabetes Mellitus, Non-insulin-dependent
• Mood Disorders

Interacting Proteins

• AKT1
• AKT2
• APP
• AXIN1
• Axin1

• BICD1
• CTNNB1
• Ctnnb1
• DAB2IP
• DACT1

• DEAF1
• Disc1
• EIF2B5
• FRAT1
• GYS1

• LRP6
• LRRK2
• Mapk1
• MAPT
• MYC

• NBR1
• NIN
• PRKACA
• RELB
• SMAD1

• SNAI1
• SNCA
• STYK1
• TP53
• TRIM29

• UBR5
• YWHAZ
• ZFPM1

Pathways

• Pathogenesis
• Cell Proliferation
• Neurogenesis
• Cell Cycle
• Localization
• Cell Death
• Secretion
• Cell Adhesion
• Axon Guidance
• S Phase
• Hyperphosphorylation
• Gene Silencing
• Brain Development

• Translation
• System Development
• Meiosis
• Neuroprotection
• Nervous System Development
• Cell Growth
• Progesterone Secretion

PTMs

• Phosphorylation
• Ubiquitination
• Reduction

• Cleavage
• Glycosylation
• Oxidation

Research Areas

• Cancer
• Hypoxia
• Lipid and Metabolism
• mTOR Pathway
• Protein Kinase

• Signal Transduction
• Wnt Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GSK3B

Boster Bio: Best Uses Of The GSK3B Marker

The Boster Bio: Best Uses Of The Human GSK3B Marker provides educators with an abundance of free educational resources. The PDFs provide the resources. These resources may be used in your teaching materials. However, it is necessary to link to the Boster Biography website or cite it. This information will be helpful to anyone interested in learning about GSK3B.

GSK-3B inhibits cancer migration

GSK-3B inhibitors of tumor cell growth have been studied in vivo and in vitro. GSK-3b inhibits chordoma cell growth by downregulating P21. Antibody-based studies used SMCC7721 cell lines and siRNAs in order to monitor their proliferation, migration, and death. GSK-3b and 21 expressions were determined by immunofluorescence as well as qRTPCR.

GSK-3B inhibits HCC cells from disassociating, allowing them to migrate into another part of the body in laboratory experiments. They also suppress Wnt signaling and prevent a cell from dissociating. These inhibitors work by inhibiting cell migration by blocking the Wnt/b-catenin signaling pathway. The long-lasting effects of these inhibitors on tumor cells are consistent with clinical studies in humans.

Inhibitors of GSK-3B inhibit tumor cell migration and metastatic ability in HCC cell lines. The inhibitors prevented cell growth in 80 HCC surgical specimens. These impressive results support the conclusion GSK-3B inhibitors might be effective in treating HCC. The clinical trial is ongoing, but preliminary results are encouraging. They have demonstrated significant effects on tumor cell migration. Boster Bio's GSK-3B inhibitions are also highly effective and inhibit tumor migration.

To examine the interaction between GSK-3b (and P21) a co-IP test was performed. Transfected cells were given si-GSK-3b for 48 hrs. Cell culture medium was mixed with the CCK-8 reagent. After four to five hours, cells were analysed by measuring absorbance at 405 nm. These values were used as the basis to plot a proliferation curve. This was used again to identify GSK-3b inhibitors in other cell types.

It is a great tool for controlling your environment

Boster Bio GSK3B is a great control for biological samples. Many biological assays utilize antibodies against GSK3A. These antibodies can either be monoclonal (or polyclonal) and react with GSK3A on many animal samples. Boster Bio has developed antibodies to GSK3A specific for rabbit and mouse.

It is a high-specificity ELISA kit

ELISA is a test used to detect an antigen in a sample. It works by binding the antigen's epitope, a protein that a normal human antibody can recognize. The amount of antigen that a particular antibody binds to determines its specificity and sensitivity. An ELISA kit allows you to determine the concentration and type of an antigen. The detection method relies on the reaction between the antigen and an enzyme/substrate, and the results are temporary. It can be used on many samples to determine whether the result is positive or negative.

The ELISA protocol was created to measure the amount of antibodies in complex mixtures. This test is now a standard for quantitative analyses of biological samples. The ELISA protocol provides versatility in the detection of proteins and peptides. There was a limited number of immunoassays before ELISA kits. These methods have been improved over time and are now the gold standard for quantification.

Five commercial CE-marked ELISA Kits for detection anti-SARS-CoV-2 IgG Antibodies were recently evaluated in a study. The specificity and sensitivity were determined using samples from patients with COVID-19. Cross-reactivity between the kits was evaluated on healthy blood donors. Overall agreement and Cohen’s Kappa were measured. As well as positive predictive scores. The assays' specificity, sensitivity, and sensitivity increased with increasing time between symptoms appearing.

Using matched pairs for detection and capture antibodies can significantly increase the sensitivity and specificity a sandwich ELISA kit. Different nonoverlapping epitopes should be recognized by both the capture and detection antibodies. Using the same antibodies limits the sensitivity and dynamic range of the ELISA. For sandwich ELISA, matched pairs are used. The primary antibodies can be obtained from primary antibody suppliers. However, it is important to confirm that they recognize the epitopes.

It is a good method of controlling the environment

Boster bio's GSK3B marker recognizes GSK3B expressing proteins in human and mouse. The GSK3B marker was validated using known positive and negative samples. The GSK3B-antiserum has also been tested in Immunofluorescence. The bosterbio GSK3Bantiserum provides a good control in GSK3B research.