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- Table of Contents
GSK3B antibody (A00791-3) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IF analysis of GSK3B using anti-GSK3B antibody (A00791-3).
GSK3B was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-GSK3B Antibody (A00791-3) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
GSK3B was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-GSK3B Antibody (A00791-3) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Designing a Western blot or IHC for GSK3B? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-GSK3 beta/GSK3B Antibody Picoband®
GSK3B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
GSK3B antibody was positively detected by WB in:
human U-87MG whole cell, human Hela whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse RAW264.7 whole cel, mouse Neuro-2a whole cel
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GSK3B antibody was positively detected by IHC in:
human breast cancer tissue, human ovarian cancer tissue, human endometrial adenocarcinoma tissue, human spleen tissue, mouse brain tissu, rat brain tissue, mouse kidney tissue, human appendix tissue, human stomach cancer tissue, human endometrial cancer tissue, human colon cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GSK3B antibody was positively detected by ICC/IF in:
U2OS cell
GSK3B antibody was positively detected by IF in:
human appendix tissue, rat testis tissue, mouse kidney tissue, human stomach cancer tissue, human colon cancer tissue, human pancreas cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Tested
Tested
Recommend
Mouse
Tested
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GSK3 beta/GSK3B Antibody Picoband®
View all GSK3B Antibodies
View all GSK3B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GSK3 beta/GSK3B recombinant protein (Position: M1-T420).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
46 kDa
Calculated Molecular Weight
46.7 kDa
Background of GSK3B
Glycogen synthase kinase-3 beta, (GSK-3 beta), is an enzyme that in humans is encoded by the GSK3B gene. The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.
Cite This Product
Anti-GSK3 beta/GSK3B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00791-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
100%
High Homology
Mouse
99%
High Homology
Rat
99%
High Homology
Sheep
99%
High Homology
Zebrafish
95%
High Homology
Cow
94%
High Homology
Drosophila
75%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GSK3B antibody
Immunohistochemistry (IHC) protocol for GSK3B antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for GSK3B antibody
Immunofluorescence (IF) protocol for GSK3B antibody
Publications
Application:
Species:
STEAP1 Suppresses Oral Squamous Cell Carcinoma by Targeting Wnt/β‐Catenin Signalling and EMT
Published: 2026-04-27
Journal: Cancer Medicine
Application: WB
Impact Factor: 3.10
Species: Human
PMID: 42046243
IER3 Promotes Non-Small Cell Lung Cancer Malignancy by Suppressing Ferroptosis via the AKT/GSK3β/NRF2 Pathway
Published: 2026-01-23
Journal: FREE RADICAL BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 8.20
Species: Human
PMID: 41581578
Effects of bergapten-loaded long-circulating liposomes on osteogenic differentiation of human dental pulp stem cells
Published: 2025-10-29
Journal: JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
Application: WB
Impact Factor: 1.30
Species: Human
PMID: 41160646
Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study
Published: 2025-02-27
Journal: Pharmaceuticals
Application: WB
Impact Factor: 4.80
Species: Human
PMID: 40143123
Naringin Protects against Tau Hyperphosphorylation in Aβ25–35-Injured PC12 Cells through Modulation of ER, PI3K/AKT, and GSK-3β Signaling Pathways
Published: 2023-02-15
Journal: BEHAVIOURAL NEUROLOGY
Application: WB
Impact Factor: 2.80
Species: Rat
PMID: 36844418
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Product Promise
We promise Anti-GSK3 beta/GSK3B Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00791-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 23, 2026