Product Info Summary
SKU: | A00791-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-GSK3 beta/GSK3B Antibody Picoband™
View all GSK-3 beta Antibodies
SKU/Catalog Number
A00791-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GSK3 beta/GSK3B Antibody Picoband™ catalog # A00791-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GSK3 beta/GSK3B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00791-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GSK3 beta/GSK3B recombinant protein (Position: M1-T420).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00791-3 is reactive to GSK3B in Human
Applications
A00791-3 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
46 kDa
Calculated molecular weight
46.744kDa
Background of GSK-3 beta
Glycogen synthase kinase-3 beta, (GSK-3 beta), is an enzyme that in humans is encoded by the GSK3B gene. The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 beta/GSK3B antigen affinity purified polyclonal antibody (Catalog # A00791-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3 beta/GSK3B at approximately 46 kDa. The expected band size for GSK3 beta/GSK3B is at 46 kDa.
Click image to see more details
Figure 2. IHC analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3).
GSK3 beta/GSK3B was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSK3 beta/GSK3B Antibody (A00791-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3).
GSK3 beta/GSK3B was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSK3 beta/GSK3B Antibody (A00791-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GSK3B (Source: Uniprot.org, NCBI)
Gene Name
GSK3B
Full Name
Glycogen synthase kinase-3 beta
Weight
46.744kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform GSK3B glycogen synthase kinase 3 beta glycogen synthase kinase-3 beta|GSK-3 beta|serine/threonine-protein kinase GSK3B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSK3B, check out the GSK3B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSK3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GSK3 beta/GSK3B Antibody Picoband™ (A00791-3)
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