Anti-GSK3 beta Rabbit Monoclonal Antibody

Western blot analysis of GSK3 beta using anti-GSK3 beta antibody (M00791).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 beta antigen affinity purified monoclonal antibody (M00791) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSK3 beta at approximately 47 kDa. The expected band size for GSK3 beta is at 47 kDa.

WECP activated Akt/GSK3β/β-Catenin signaling pathway in DPCs. DPCs were incubated with WECP (40, 80 and 160 μg/mL) in culture medium for 24 h. (A) Akt and GSK3β phosphorylation levels were detected in DPCs after WECP treatment. (B) Effects of WECP treatment on β-Catenin and Wnt10b protein expression levels in DPCs. (C) Transcriptional expression of LEF1, IGF1, and VEGF in DPCs detected using RT-PCR. Data are presented as means ± SD of three independent replicates. * p < 0.05, ** p < 0.01 vs. control group.
Index in PubMed under a CC BY license. PMID: 36891275

WECP activated Akt/GSK3β/β-Catenin signaling pathway in denuded mouse skin. (A) Effects of WECP and finasteride treatments on Akt and GSK3β protein phosphorylation in mouse skin. (B) Effects of WECP and finasteride treatments on β-Catenin and Wnt10 translational expression in mouse skin. (C) Effects of WECP and finasteride treatments on transcriptional expression of β-Catenin, Wnt10b, Wnt5a, LEF1, VEGF, and IGF1 in mouse skin. Data are means ± SD of three independent replicates. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group. WECPL, WECPH, and Fin represent low-dose WECP, high-dose WECP, and finasteride treatment, respectively.
Index in PubMed under a CC BY license. PMID: 36891275

Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Human small cell lung cancer , using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Human pancreatic cancer, using the Antibody at 1:400 dilution.

Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded human colon carcinome, using GSK3 beta Antibody.

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Immunofluorescent analysis of Hela cells, using GSK3 beta Antibody .