Anti-HO-1 Monoclonal Antibody (HO-1-2)

Product Name

Anti-HO-1 Monoclonal Antibody (HO-1-2)

View all HO-1/HMOX1/HSP32 Antibodies

SKU/Catalog Number

M00253-1

Size

50 µg

Form

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Description

Boster Bio Anti-HO-1 Monoclonal Antibody (HO-1-2) catalog # M00253-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HO-1 Monoclonal Antibody (HO-1-2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00253-1)

Host

Mouse

Contents

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Clonality

Monoclonal

Clone Number

HO-1-2

Isotype

IgG

Immunogen

Native rat HO-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00253-1 is reactive to HMOX1 in Human, Mouse, Rat

Applications

M00253-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

32.819kDa

Background of HO-1/HMOX1/HSP32

Anti Apolipoprotein C-III antibody recognizes the gene product of APOC. Apolipoprotein C-III is a protein component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to inhibit hepatic uptake of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. This antibody is suitable for cardiovascular research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Flow cytometry analysis of human lung cancer A2 cells stained using isotype control antibody (left) and HO-1 (Hsp32) mAb (HO -1-2) (right).

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Figure 1. Western blot analysis of Hmox1 using anti-Hmox1 antibody (M00253-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hmox1 antigen affinity purified polyclonal antibody (Catalog # M00253-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Hmox1.

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Figure 3. IHC analysis of Hmox1 using anti-Hmox1 antibody (M00253-1).
Hmox1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Hmox1 Antibody (M00253-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

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Figure 4. IHC analysis of Hmox1 using anti-Hmox1 antibody (M00253-1).
Hmox1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Hmox1 Antibody (M00253-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Gene/Protein Information For HMOX1 (Source: Uniprot.org, NCBI)

Alternative Names

bK286B10; EC 1.14.99.3; heat shock protein, 32-kD; heme oxygenase (decycling) 1; HMOX1; HO; HO1; HO-1; HO-1heme oxygenase 1; HSP32 HMOX1 HMOX1D, HO-1, HSP32, bK286B10 heme oxygenase 1 heme oxygenase 1|heat shock protein, 32-kD|heme oxygenase (decycling) 1

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