Anti-HO-1 Monoclonal Antibody (HO-1-2)

HO-1/HMOX1/HSP32 antibody

Boster Bio Anti-HO-1 Monoclonal Antibody (HO-1-2) catalog # M00253-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 19 publication(s).

Product Info Summary

SKU: M00253-1
Size: 1mg
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IHC, WB

Product Name

Anti-HO-1 Monoclonal Antibody (HO-1-2)

View all HO-1/HMOX1/HSP32 Antibodies

SKU/Catalog Number





Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.


Boster Bio Anti-HO-1 Monoclonal Antibody (HO-1-2) catalog # M00253-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HO-1 Monoclonal Antibody (HO-1-2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00253-1)




Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.



Clone Number



Native rat HO-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00253-1 is reactive to Hmox1 in Human, Mouse, Rat


M00253-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

Gene/Protein Information For Hmox1 (Source: Uniprot.Org, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

Heme oxygenase 1




heme oxygenase family

Alternative Names

bK286B10; EC; heat shock protein, 32-kD; heme oxygenase (decycling) 1; HMOX1; HO; HO1; HO-1; HO-1heme oxygenase 1; HSP32 Hmox1|HEOXG, Heox, Hmox, Ho-1, Ho1, hsp32|heme oxygenase 1|heme oxygenase 1|heme oxygenase (decycling) 1|heme oxygenase (decyclizing) 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Hmox1, check out the Hmox1 Infographic

Hmox1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Hmox1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00253-1 has been cited in 19 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Dong H,Hao L,Zhang W,Zhong W,Guo W,Yue R,Sun X,Zhou Z.Activation of AhR-NQO1 signaling pathway protects against alcohol-induced liver injury by improving redox balance.Cell Mol Gastroenterol Hepatol.2021 May 31:S2352-345X(21)00102-8.doi:10.1016/j.jcmgh.2021.05.013.Epub ahead of print.PMID:34082111.
Species: Human,Mouse

Sun S,Zhang J,Li H,Du Y,Li S,Li A,Suo X,Wang Y,Sun Q.Anti-inflammatory activity of the water extract of Chloranthus serratus roots in LPS-stimulated RAW264.7 cells mediated by the Nrf2/HO-1, MAPK and NF-κB signaling pathways.J Ethnopharmacol.2021 Jan 25:1
Species: Mouse
M00253-1 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:1:400

Li D,Bai X,Jiang Y,Cheng Y.Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway.Brain Res Bull.2020 Dec 28;168:25-35.doi:10.1016/j.brainresbull.2020.12.009.Epub ahead of print
Species: Mouse
M00253-1 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS, DILUTION:1:200

Sun S,Wang Y,Du Y,Sun Q,He L,Zhu E,Li J. Oxidative stress-mediated hepatotoxicity in rats induced by ethanol extracts of different parts of Chloranthus serratus.Pharm Biol.2020 Dec;58(1):1277-1289.doi:10.1080/ n13880209.2020.1859552.PMID: 33355514.
Species: Rat
M00253-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:400

Role of heme oxygenase-1/carbon monoxide system in pulmonary ischemia-reperfusion injury

Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats

Endogenous carbon monoxide downregulates hepatic cystathionine-?-lyase in rats with liver cirrhosis

Hydrogen sulfide accelerates wound healing in diabetic rats

Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression

Evidence for a therapeutic effect of Braintone on ischemic brain damage

Upregulation of heat shock protein 32 in Sertoli cells alleviates the impairments caused by heat shock-induced apoptosis in mouse testis

Effect of heme oxygenase-1 on renal function in rats with liver cirrhosis

Influence of heme oxygenase-1 expression on immune liver fibrosis induced by cobalt protoporphyrin in rats

Changes of hypoxia-inducible factor-1 signaling and the effect of cilostazol in chronic cerebral ischemia

Comparison of the Effects of Propofol and Midazolam on Inflammation and Oxidase Stress in Children with Congenital Heart Disease Undergoing Cardiac Surgery

Wang G, Li W, Lu X, Zhao X. Heart Int. 2011 Sep 29;6(2):E21. Doi: 10.4081/Hi.2011.E21. Epub 2011 Nov 22. Riboflavin Alleviates Cardiac Failure In Type I Diabetic Cardiomyopathy.

Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...

Mo L, Yang C, Gu M, Zheng D, Lin L, Wang X, Lan A, Hu F, Feng J. Int J Mol Med. 2012 Aug;30(2):314-20. Doi: 10.3892/Ijmm.2012.1002. Epub 2012 May 14. Pi3K/Akt Signaling Pathway-Induced Heme Oxygenase-1 Upregulation Mediates The Adaptiv...

Wang Gg, Li W, Lu Xh, Zhao X, Xu L. Croat Med J. 2013 Apr;54(2):171-9. Taurine Attenuates Oxidative Stress And Alleviates Cardiac Failure In Type I Diabetic Rats.

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5 Customer Q&As for Anti-HO-1 Monoclonal Antibody (HO-1-2)


We have been able to see staining in rat layer of synovial tissue. Any tips? Is anti-HO-1 Monoclonal antibody (HO-1-2) supposed to stain layer of synovial tissue positively?

Verified Customer

Verified customer

Asked: 2020-03-06


According to literature layer of synovial tissue does express HMOX1. According to, HMOX1 is expressed in layer of synovial tissue, foreskin, cervix carcinoma, among other tissues. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585

Boster Scientific Support

Answered: 2020-03-06


My team were satisfied with the WB result of your anti-HO-1 Monoclonal antibody (HO-1-2) . However we have been able to see positive staining in layer of synovial tissue microsome. using this antibody. Is that expected? Could you tell me where is HMOX1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-03-22


Based on literature, layer of synovial tissue does express HMOX1. Generally HMOX1 expresses in microsome. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585

Boster Scientific Support

Answered: 2019-03-22


I would like using your anti-HO-1 Monoclonal antibody (HO-1-2) for regulation of dna-binding transcription factor activity studies. Has this antibody been tested with western blotting on cancer a2 cells? We would like to see some validation images before ordering.

A. Taylor

Verified customer

Asked: 2018-06-26


Thanks for your inquiry. This M00253-1 anti-HO-1 Monoclonal antibody (HO-1-2) is tested on cancer a2 cells. It is guaranteed to work for Flow Cytometry, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-06-26


We are currently using anti-HO-1 Monoclonal antibody (HO-1-2) M00253-1 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

A. Huang

Verified customer

Asked: 2018-05-31


The anti-HO-1 Monoclonal antibody (HO-1-2) (M00253-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-31


We bought anti-HO-1 Monoclonal antibody (HO-1-2) for Flow Cytometry on foreskin in the past. I am using human, and We intend to use the antibody for IHC next. I am looking for examining foreskin as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2017-09-19


I have checked the website and datasheets of our anti-HO-1 Monoclonal antibody (HO-1-2) and I see that M00253-1 has been tested on human in both Flow Cytometry and IHC. Thus M00253-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website to find out more information about them.

Boster Scientific Support

Answered: 2017-09-19


Total: $343


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