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- Table of Contents
Designing a Western blot for Krueppel-like factor 3? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the KLF3 Western Blot GuideFacts about Krueppel-like factor 3.
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| Mouse | |
|---|---|
| Gene Name: | Klf3 |
| Uniprot: | Q60980 |
| Entrez: | 16599 |

| Belongs to: |
|---|
| krueppel C2H2-type zinc-finger protein family |

Basic krueppel-like factor; basic Kruppel-like factor; BKLFbasic kruppel like factor; CACCC-box-binding protein BKLF; Krueppel-like factor 3; Kruppel-like factor 3 (basic); MGC48279; TEF-2
Mass (kDA):
38.561 kDA

| Mouse | |
|---|---|
| Location: | 5|5 C3.1 |
| Sequence: | 5; |
In 8.5 day embryos, expressed in midbrain, anterior hindbrain and ventral forebrain. In 9 day embryos, expressed throughout ventral anterior half of embryo including midbrain-hindbrain junction, ventral midbrain, diencephalon and forebrain. At 10.5 days, distribution is more widespread with expression also found in developing limb buds. Widely expressed in the adult.



PMID: 8657145 by Crossley M., et al. Isolation and characterization of the cDNA encoding BKLF/TEF-2, a major CACCC-box-binding protein in erythroid cells and selected other cells.
PMID: 15684403 by Perdomo J., et al. Role for SUMO modification in facilitating transcriptional repression by BKLF.