MADD Antibodies

AND MADD ELISA kits, MADD Proteins

Many biological assays use antibodies to detect MADD, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with MADD in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop MADD antibodies...

We validate the specificity of these antibodies to MADD by testing them on tissues known to express MADD positively and negatively. Browse below to find the MADD antibody that suites your experiment. We have 5 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for MADD? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the MADD Western Blot Guide

MADD Infographic by Boster Bio

All MADD Products

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Product

Figures

Specificity

Applications

Price

Anti-MADD Antibody Picoband®

Western blot analysis of MADD using anti-MADD antibody (A02030-1)

Flow Cytometry analysis of Hela cells using anti-MADD antibody (A02030-1)

Human, Mouse, Rat

ELISA, Flow Cytometry, WB

$370
Cat # A02030-1
100 μg/vial

Anti-Zebrafish MADD Antibody Picoband®

Western blot analysis of MADD using anti-MADD antibody (AZA0A8M3AUB1)

Zebrafish

$470
Cat # AZA0A8M3AUB1
100 μg/vial

Anti-DENN Rabbit Monoclonal Antibody

Western blot analysis of DENN expression in HeLa cell lysate

Human, Mouse, Rat

IHC, WB

$370
Cat # M02030
100 μl/vial

MADD Colorimetric Cell-Based ELISA

Western blot analysis of extracts from COLO/HeLa/Jurkat cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1886
1 kit, containing one 96-well plate and all necessary reagents

DENN (MADD) (NM_003682) Human Over-expression Lysate

Human

$960
Cat # LS008567
20 µg, 100 µg

MADD Overview

Facts about MAP kinase-activating death domain protein.

MADD 3D structure. Source: alphafold

MAP kinase-activating death domain protein (MADD)

Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows diminished.

Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Part of the TNFRSF1A signaling complex: MADD connections TNFRSF1A with MAP kinase activation.

Gene Information

Human
Gene Name:	MADD
Uniprot:	Q8WXG6
Entrez:	8567

Family

Belongs to:

MADD family

Alternative Names

MAP kinase-activating death domain protein

Molecular Weight

Mass (kDA):
183.303 kDA

Genomic Context


Human

Location:	11p11.2
Sequence:	11; NC_000011.10 (47269188..47330031)

Tissue Specificity

Highly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines.

Subcellular Localizations

Membrane. Cytoplasm.

Interacting Proteins

POT1
PTPRN

PTMs

Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MADD

References

PMID: 8988362 by Chow V.T.K., et al. DENN, a novel human gene differentially expressed in normal and neoplastic cells.

PMID: 9115275 by Schievella A.R., et al. MADD, a novel death domain protein that interacts with the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase.