NDUFB4 Antibodies

AND NDUFB4 ELISA kits, NDUFB4 Proteins

Many biological assays use antibodies to detect NDUFB4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with NDUFB4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop NDUFB4 antibodies...

We validate the specificity of these antibodies to NDUFB4 by testing them on tissues known to express NDUFB4 positively and negatively. Browse below to find the NDUFB4 antibody that suites your experiment. We have 5 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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NDUFB4 Infographic by Boster Bio

All NDUFB4 Products

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Applications

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Anti-NDUFB4 Antibody

Western blot analysis of extracts of various cell lines, using NDUFB4 antibody at 1:3000 dilution

Immunohistochemistry of paraffin-embedded human liver using [KO Validated] NDUFB4 Rabbit pAb at dilution of 1:150

Western blot analysis of extracts from normal and NDUFB4 knockout HeLa cells, using NDUFB4 antibody at 1:1000 dilution

Human, Mouse, Rat

IF, IHC, ICC, WB

$405
Cat # A11016
100ul

Human NDuFB4 Recombinant Protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

SDS-PAGE, Denatured

$250
Cat # PROTO95168
Starting from 20ug

Anti-NDUFB4 Rabbit Monoclonal Antibody

Western blot analysis of NDUFB4 using anti-NDUFB4 antibody (M11016)

Human, Mouse, Rat

IF, IHC, ICC, WB

$370
Cat # M11016
100 μl/vial

NDUFB4 (NM_004547) Human Over-expression Lysate

Human

$628
Cat # LS004710
20 µg, 100 µg

Anti-NDUFB4 Antibody Picoband®

Western blot analysis of NDUFB4 using anti-NDUFB4 antibody (A11016-1)

IF analysis of NDUFB4 using anti-NDUFB4 antibody (A11016-1)

Flow Cytometry analysis of THP-1 cells using anti-NDUFB4 antibody (A11016-1)

Human, Mouse, Rat

ELISA, Flow Cytometry, IF, ICC, WB

$370
Cat # A11016-1
100 µg/vial

NDUFB4 Overview

Facts about NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4.

NDUFB4 3D structure. Source: alphafold

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (NDUFB4)

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain.

The immediate electron acceptor for the enzyme is believed to be ubiquinone. .

Gene Information

Human
Gene Name:	NDUFB4
Uniprot:	O95168
Entrez:	4710

Family

Belongs to:

complex I NDUFB4 subunit family

Alternative Names

B15; CI-B15; complex I B15 subunit; Complex I-B15; MGC5105; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4 (15kD, B15); NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; NADH-ubiquinone oxidoreductase B15 subunit

Molecular Weight

Mass (kDA):
15.209 kDA

Genomic Context


Human

Location:	3q13.33
Sequence:	3; NC_000003.12 (120596336..120602507)

Subcellular Localizations

Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.

Diseases

Malignant Neoplasms
Non-small Cell Lung Carcinoma
Neurodegenerative Disorders
Nerve Degeneration
Malignant Neoplasm Of Lung
Parkinson Disease

Pathways

Oxidative Phosphorylation
Cell Death
Aging

PTMs

Nitration

Oxidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NDUFB4

References

PMID: 9878551 by Loeffen J.L.C.M., et al. cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed.

PMID: 27626371 by Stroud D.A., et al. Accessory subunits are integral for assembly and function of human mitochondrial complex I.