Nuclear factor 1 X-type Antibodies

AND NFIX ELISA kits, Nuclear factor 1 X-type Proteins

Many biological assays use antibodies to detect Nuclear factor 1 X-type, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Nuclear factor 1 X-type in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Nuclear factor 1 X-type antibodies...

We validate the specificity of these antibodies to Nuclear factor 1 X-type by testing them on tissues known to express NFIX positively and negatively. Browse below to find the NFIX antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for Nuclear factor 1 X-type? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the NFIX Western Blot Guide

NFIX Infographic by Boster Bio

All NFIX Products

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Specificity

Applications

Price

Anti-NFIX Antibody Picoband®

Western blot analysis of NFIX using anti-NFIX antibody (A04138-1)

IF analysis of NFIX using anti-NFIX antibody (A04138-1)

Flow Cytometry analysis of U20S cells using anti-NFIX antibody (A04138-1)

Human, Mouse, Rat

ELISA, Flow Cytometry, IF, ICC, WB

$370
Cat # A04138-1
100 µg/vial

NFIX (NM_002501) Human Over-expression Lysate

Human

$960
Cat # LS004784
20 µg, 100 µg

NFIX Overview

Facts about Nuclear factor 1 X-type.

NFIX 3D structure. Source: alphafold

Nuclear factor 1 X-type (NFIX)

Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Gene Information

Human
Gene Name:	NFIX
Uniprot:	Q14938
Entrez:	4784

Family

Belongs to:

CTF/NF-I family

Alternative Names

CTF; NF1ACCAAT-box-binding transcription factor; NF1-X; NF-I/X; NFI-X; nuclear factor 1 X-type; Nuclear factor 1/X; nuclear factor I/X (CCAAT-binding transcription factor); Nuclear factor I/X; TGGCA-binding protein

Molecular Weight

Mass (kDA):
55.098 kDA

Genomic Context


Human

Location:	19p13.13
Sequence:	19; NC_000019.10 (12995475..13098796)

Tissue Specificity

Widely expressed.

Subcellular Localizations

Nucleus.

Diseases

Neoplasms
Hydrocephalus
Congenital Absence
Mammary Neoplasms
Glioma
Macrocephaly
Nervousness
Developmental Delay (disorder)
Glioblastoma
Haploinsufficiency
Disease Of Adrenal Medulla
Teratoma

Communicating Hydrocephalus
Malignant Neoplasm Of Breast
Carcinogenesis
Osteosarcoma
Congenital Hydrocephalus
Congenital Anomaly Of Brain

Interacting Proteins

QRICH1
STAT3
ZNF614

Pathways

Dna Replication
Brain Development
Axon Guidance
Metaphase
Localization
Methylation
Cell Adhesion
Cell Migration
Developmental Process
Dna Demethylation
Neurogenesis
Regulation Of Gene Expression
Ossification

Mesenchymal Cell Proliferation
Cell Cycle
Histone Acetylation
Cell Differentiation
Epithelial Cell Proliferation
Bone Mineralization
Endochondral Ossification

PTMs

Methylation
Demethylation

Phosphorylation
Acetylation

Research Areas

Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NFIX

References

PMID: 7937100 by Apt D., et al. Cloning and functional analysis of spliced isoforms of human nuclear factor I-X: interference with transcriptional activation by NFI/CTF in a cell-type specific manner.

PMID: 8799200 by Sumner C., et al. Expression of multiple classes of the nuclear factor-1 family in the developing human brain: differential expression of two classes of NF-1 genes.