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- Table of Contents
NUP210 · Western blot design guide
NUP210 Western Blot Planning Guide
Plan a NUP210 Western blot around the catalog-observed 205.1 kDa band, image-backed A05308 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
NUP210 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 205.1 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 4-12% gradient | |
| Positive control | Lung | |
| Negative control | Heart muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 205.1 kDa |
| ⓘ | Localization | Nucleus, nuclear pore complex / Nucleus membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Rat |
Section 1
Real Curated NUP210 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Lung |
| Gel % | 4-12% gradient |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A05308 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected NUP210 Western Blot Band Size?
Use the product-observed 205.1 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 205.1 kDa | Matches the authoritative product WB observation. |
| 205.1 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected NUP210 appearancePlan around 205.1 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 205.1 kDa; use this as the primary experimental expectation. |
| Calculated mass | 205.1 kDa from UniProt Q8TEM1; retain as context. |
| Gel selection | 4-12% gradient; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A05308. |
| Situation | Likely cause | Next action |
|---|---|---|
| 205.1 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing NUP210 band
A05308 · NUP210
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for NUP210 Western blot
🧪Use Lung as the first positive-control candidate and Heart muscle as the HPA Not detected negative candidate.
Positive control: Lung (High)
Negative control: Heart muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for NUP210
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Lung | Reported tissue cells | High | Protein (HPA) | HPA → |
| Prostate | Reported tissue cells | High | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced NUP210 Western Blot Tips
Deeper troubleshooting and optimisation questions for NUP210, answered from its protein features.
Which band should guide the blot?
Use 205.1 kDa, the observation attached to the authoritative A05308 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 205.1 kDa expectation.
Which positive control should I start with?
Start with Lung, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Heart muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 4-12% gradient consistently across the quick facts, protocol table, and poster.
What transfer method to use for NUP210 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A05308 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC8669001, PMC5976539, PMC7214106.
Boster reagents
NUP210 Western Blot Reagents
Human/Rat-reactive NUP210 Western blot reagents with authoritative product imagery.
Real WB data
Anti-GP210/NUP210 Antibody Picoband®
Cat # A05308
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q8TEM1
- Human Protein Atlas — NUP210
- A05308 product record
- PMC8669001 — Nuclear pore protein NUP210 depletion suppresses metastasis through heterochromatin-mediated disruption of tumor cell mechanical response.
- PMC5976539 — Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4(+) T cell homeostasis.
- PMC7214106 — NUP210 and MicroRNA-22 Modulate Fas to Elicit HeLa Cell Cycle Arrest.