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- Table of Contents
PAM Antibodies
AND PAM ELISA kits, PAM Proteins
Many biological assays use antibodies to detect PAM, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with PAM in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop PAM antibodies...
Designing a Western blot for PAM? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PAM Western Blot GuideDesigning an IHC experiment for PAM? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the PAM IHC GuidePAM Infographic by Boster Bio
All PAM Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, Flow Cytometry, IHC, WB
$370
Cat # A00332-2
100 μg/vial
Cat # A00332-2
100 μg/vial
Human, Mouse, Rabbit, Rat
ELISA, Flow Cytometry, Functional Assay, Kinetics
$486
Cat # M29279
100 μg
Cat # M29279
100 μg
PAM Overview
Facts about Peptidyl-glycine alpha-amidating monooxygenase.
Peptidyl-glycine alpha-amidating monooxygenase (PAM)
Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that's dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part.
C-terminal amidation of peptides such as neuropeptides is essential for full biological activity. .
Gene Information
| Human | |
|---|---|
| Gene Name: | PAM |
| Uniprot: | P19021 |
| Entrez: | 5066 |
Family
| Belongs to: |
|---|
| No superfamily |
Alternative Names
PAL; PAM; pancreatic peptidylglycine alpha-amidating monooxygenase; peptidyl alpha-amidating enzyme; peptidyl-alpha-hydroxyglycine alpha-amidating lyase; peptidylamidoglycolate lyase; peptidylglycine 2-hydroxylase; peptidyl-glycine alpha-amidating monooxygenase; peptidylglycine alpha-amidating monooxygenase; peptidylglycine alpha-hydroxylating monooxygenase; PHM
Molecular Weight
Mass (kDA):
108.332 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 5q21.1 |
| Sequence: | 5; NC_000005.10 (102754783..103031105) |
Subcellular Localizations
Cytoplasmic vesicle, secretory vesicle membrane; Single-pass membrane protein. Secretory granules.; [Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted. Secreted from secretory granules.; [Isoform 4]: Secreted. Secreted from secretory granules.
Diseases
- Neoplasms
- Malignant Neoplasms
- Inflammation
- Pituitary Diseases
- Poisoning
- Carcinoma
- Nervousness
- Infective Disorder
- Malignant Paraganglionic Neoplasm
- Tissue Adhesions
- Melanoma
- Pneumonia
- Neoplasm Metastasis
Pathways
- Secretion
- Phagocytosis
- Transport
- Immune Response
- Localization
- Pathogenesis
- Photosynthesis
- Cell Proliferation
- Inflammatory Response
- Cell Growth
- Chemotaxis
- Electron Transport
- Flocculation
PTMs
- Amidation
- Phosphorylation
- Cleavage
- Hydroxylation
- Oxidation
- Reduction
- Glycosylation
- Iodination
- Ubiquitination
- Biotinylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PAM
bosterbio.com/bosterbio-gene-info-cards/PAM
References
PMID: 2357221 by Glauder J., et al. Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells.
PMID: 7999037 by Tateishi K., et al. Isolation and functional expression of human pancreatic peptidylglycine alpha-amidating monooxygenase.