POLR2D Antibodies

AND POLR2D ELISA kits, POLR2D Proteins

Many biological assays use antibodies to detect POLR2D, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with POLR2D in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop POLR2D antibodies...

We validate the specificity of these antibodies to POLR2D by testing them on tissues known to express POLR2D positively and negatively. Browse below to find the POLR2D antibody that suites your experiment. We have 5 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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POLR2D Infographic by Boster Bio

All POLR2D Products

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Product

Figures

Specificity

Applications

Price

Anti-POLR2D Antibody

Western blot analysis of extracts of various cell lines, using POLR2D antibody at 1:1000 dilution

Immunofluorescence analysis of A549 cells using POLR2D antibody

Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug POLR2D antibody

Human, Mouse

IP, IF, ICC, WB

$405
Cat # A12839
100ul

Human POLR2D Recombinant Protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

SDS-PAGE, Denatured

$250
Cat # PROTO15514
Starting from 20ug

POLR2D (NM_004805) Human Over-expression Lysate

Human

$628
Cat # LS005433
20 µg, 100 µg

Anti-POLR2D Antibody Picoband®

Western blot analysis of POLR2D using anti-POLR2D antibody (A12839-1)

Human, Mouse, Rat

ELISA, WB

$370
Cat # A12839-1
100 µg/vial

Anti-Zebrafish POLR2D Antibody

IHC analysis of POLR2D using anti-POLR2D antibody (AZA0A8M1N062)

Zebrafish

IHC

$470
Cat # AZA0A8M1N062
100 μg/vial

POLR2D Overview

Facts about DNA-directed RNA polymerase II subunit RPB4.

POLR2D 3D structure. Source: alphafold

DNA-directed RNA polymerase II subunit RPB4 (POLR2D)

Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery.

It's composed of mobile elements that move relative to one another. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element.

Gene Information

Human
Gene Name:	POLR2D
Uniprot:	O15514
Entrez:	5433

Family

Belongs to:

eukaryotic RPB4 RNA polymerase subunit family

Alternative Names

DNA-directed RNA polymerase II 16 kDa polypeptide; DNA-directed RNA polymerase II subunit D; DNA-directed RNA polymerase II subunit RPB4; HSRBP4; HSRPB4; polymerase (RNA) II (DNA directed) polypeptide D; RBP4; RNA polymerase II 16 kDa subunit; RNA polymerase II subunit B4; RNA polymerase II subunit hsRBP4; RPB16

Molecular Weight

Mass (kDA):
16.311 kDA

Genomic Context


Human

Location:	2q14.3
Sequence:	2; NC_000002.12 (127843553..127858155, complement)

Subcellular Localizations

Nucleus.

Diseases

Diabetes Mellitus, Non-insulin-dependent
Glioblastoma Multiforme
Malignant Neoplasms
Obesity
Glioblastoma
Diabetes Mellitus

Pathways

Programmed Cell Death
Cell Death
Cell Cycle
Aging

Research Areas

Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/POLR2D

References

PMID: 9528765 by Khazak V., et al. Analysis of the interaction of the novel RNA polymerase II (pol II) subunit hsRPB4 with its partner hsRPB7 and with pol II.

PMID: 9852112 by Kershnar E., et al. Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes.