POLR3D Antibodies

AND POLR3D ELISA kits, POLR3D Proteins

Many biological assays use antibodies to detect POLR3D, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with POLR3D in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop POLR3D antibodies...

We validate the specificity of these antibodies to POLR3D by testing them on tissues known to express POLR3D positively and negatively. Browse below to find the POLR3D antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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POLR3D Infographic by Boster Bio

All POLR3D Products

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Anti-RPC4 POLR3D Antibody

1 Q&As

Immunohistochemical analysis of paraffin-embedded human tonsil

Western blot analysis of the lysates from HepG2 cells using RPC4 antibody

Western blot analysis of lysates from MCF-7 cells, using RPC4 Antibody

Human, Mouse

ELISA, IHC, WB

$335
Cat # A30652
100ul

POLR3D (NM_001722) Human Recombinant Protein

Coomassie blue staining of purified POLR3D protein

$1249
Cat # PROTP05423
20 µg

POLR3D (NM_001722) Human Over-expression Lysate

Human

$628
Cat # LS000661
20 µg, 100 µg

Anti-POLR3D Antibody Picoband®

Western blot analysis of POLR3D using anti-POLR3D antibody (A11611-2)

IHC analysis of POLR3D using anti-POLR3D antibody (A11611-2)

IF analysis of POLR3D using anti-POLR3D antibody (A11611-2) and anti-Beta Tubulin antibody (M01857-3)

Human, Rat

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A11611-2
100 µg/vial

POLR3D Overview

Facts about DNA-directed RNA polymerase III subunit RPC4.

POLR3D 3D structure. Source: alphafold

DNA-directed RNA polymerase III subunit RPC4 (POLR3D)

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.

Plays a vital role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune reaction.

Gene Information

Human
Gene Name:	POLR3D
Uniprot:	P05423
Entrez:	661

Family

Belongs to:

eukaryotic RPC4/POLR3D RNA polymerase subunit family

Alternative Names

BN51 (BHK21) temperature sensitivity complementing; BN51; BN51TDNA-directed RNA polymerase III 47 kDa polypeptide; DNA-directed RNA polymerase III subunit D; DNA-directed RNA polymerase III subunit RPC4; polymerase (RNA) III (DNA directed) polypeptide D, 44kDa; Protein BN51; RNA polymerase III 47 kDa subunit; RNA polymerase III subunit C4; RPC4; RPC53 homolog; temperature sensitive complementation, cell cycle specific, tsBN51; TSBN51RPC53

Molecular Weight

Mass (kDA):
44.396 kDA

Genomic Context


Human

Location:	8p21.3
Sequence:	8; NC_000008.11 (22245104..22254601)

Subcellular Localizations

Nucleus.

Pathways

Gene Silencing
Cell Cycle
Rna Interference

PTMs

Acetylation
Isopeptide bond

Methylation
Phosphoprotein

Ubl conjugation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/POLR3D

References

PMID: 3683386 by Ittmann M., et al. Isolation of the human gene that complements a temperature-sensitive cell cycle mutation in BHK cells.

PMID: 12391170 by Hu P., et al. Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.