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- Table of Contents
PSMA2 · Western blot design guide
PSMA2 Western Blot Planning Guide
Plan a PSMA2 Western blot around the catalog-observed 25.9 kDa band, image-backed PB10088 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
PSMA2 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 25.9 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Adrenal gland | |
| Negative control | Skeletal muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 25.9 kDa |
| ⓘ | Localization | Cytoplasm / Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Rat |
Section 1
Real Curated PSMA2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB10088 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected PSMA2 Western Blot Band Size?
Use the product-observed 25.9 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 25.9 kDa | Matches the authoritative product WB observation. |
| 25.9 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected PSMA2 appearancePlan around 25.9 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 25.9 kDa; use this as the primary experimental expectation. |
| Calculated mass | 25.9 kDa from UniProt P25787; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB10088. |
| Situation | Likely cause | Next action |
|---|---|---|
| 25.9 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing PSMA2 band
PB10088 · PSMA2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PSMA2 Western blot
🧪Use Adrenal gland as the first positive-control candidate and Skeletal muscle as the HPA Not detected negative candidate.
Positive control: Adrenal gland (Medium)
Negative control: Skeletal muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for PSMA2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced PSMA2 Western Blot Tips
Deeper troubleshooting and optimisation questions for PSMA2, answered from its protein features.
Which band should guide the blot?
Use 25.9 kDa, the observation attached to the authoritative PB10088 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 25.9 kDa expectation.
Which positive control should I start with?
Start with Adrenal gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Skeletal muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for PSMA2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB10088 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
PSMA2 Western Blot Reagents
Human/Rat-reactive PSMA2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-Proteasome 20S alpha 2/PSMA2 Antibody Picoband®
Cat # PB10088
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P25787
- Human Protein Atlas — PSMA2
- PB10088 product record
- PMC7118148 — Cell cycle-dependent localization of the proteasome to chromatin (Scientific reports, 2020)
- PMC8906419 — Influenza A Virus Uses PSMA2 for Downregulation of the NRF2-Mediated Oxidative Stress Response (Journal of virology, 2022)
- PMC11724067 — PSMA2 promotes chemo- and radioresistance of oral squamous cell carcinoma by modulating mitophagy pathway (Cell death discovery, 2025)
