PSMB5 · Western blot design guide

PSMB5 Western Blot Planning Guide

Plan a PSMB5 Western blot around the catalog-observed 28.5 kDa band, image-backed A03418-1 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for PSMB5 (PSMB5): expected band 28.5 kDa, antibody A03418-1, and PMC-cited SDS-PAGE protocol steps — PSMB5 Western blot protocol sheet — expected band 28.5 kDa, antibody A03418-1, controls and PMC citations. Open the full PSMB5 WB guide →

Navigate to: Protocols Band Controls Antibodies

PSMB5 Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	28.5 kDa
	Observed band	Not reported — verify product WB image
	Gel	12-15%
	Positive control ⓘ	Testis
	Negative control ⓘ	Appendix

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	28.5 kDa
ⓘ	Localization	Cytoplasm / Nucleus
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human / Mouse / Rat

Section 1

Real Curated PSMB5 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Cerebellum
Gel %	12-15%
Load	20-30 µg total protein per lane
Transfer	Semi-dry, short transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	A03418-1 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 2

What Is the Expected PSMB5 Western Blot Band Size?

Use the product-observed 28.5 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
28.5 kDa	Matches the authoritative product WB observation.
28.5 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected PSMB5 appearancePlan around 28.5 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	28.5 kDa; use this as the primary experimental expectation.
Calculated mass	28.5 kDa from UniProt P28074; retain as context.
Gel selection	12-15%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with A03418-1.

Why is my band missing or off?
Situation	Likely cause	Next action
28.5 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt P28074 Human Protein Atlas product datasheet (A03418-1)

Experiment evidence

Western blot images showing PSMB5 band

A03418-1 · PSMB5

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Sample controls for PSMB5 Western blot

🧪Use Testis as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.

Positive control: Testis (High)

Negative control: Appendix (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for PSMB5

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Testis	Reported tissue cells	High	Protein (HPA)	HPA →
Cerebellum	Reported tissue cells	High	Protein (HPA)	HPA →
Adrenal gland	Reported tissue cells	Medium	Protein (HPA)	HPA →
Caudate	Reported tissue cells	Medium	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Appendix	Reported tissue cells	Not detected	Protein (HPA)	HPA →
Bone marrow	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 3

Advanced PSMB5 Western Blot Tips

Deeper troubleshooting and optimisation questions for PSMB5, answered from its protein features.

Which band should guide the blot?

Use 28.5 kDa, the observation attached to the authoritative A03418-1 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 28.5 kDa expectation.

Which positive control should I start with?

Start with Testis, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Appendix as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 12-15% consistently across the quick facts, protocol table, and poster.

What transfer method to use for PSMB5 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should A03418-1 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC12818076, PMC11507914, PMC11896255.

Boster reagents

PSMB5 Western Blot Reagents

Human/Mouse/Rat-reactive PSMB5 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for PSMB5 using A03418-1; observed band 28.5 kDa

Anti-PSMB5/MB1 Antibody Picoband®

Cat # A03418-1

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.