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Retinoid isomerohydrolase Antibodies
AND RPE65 ELISA kits, Retinoid isomerohydrolase Proteins
Many biological assays use antibodies to detect Retinoid isomerohydrolase, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Retinoid isomerohydrolase in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Retinoid isomerohydrolase antibodies...
Designing a Western blot for Retinoid isomerohydrolase? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the RPE65 Western Blot GuideRPE65 Infographic by Boster Bio
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RPE65 Overview
Facts about Retinoid isomerohydrolase.
Retinoid isomerohydrolase (RPE65)
Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans- retinyl fatty acid esters into 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed:16116091).
Also capable of catalyzing the isomerization of lutein into meso-zeaxanthin an eye-specific carotenoid (PubMed:28874556). The soluble form binds vitamin A (all-trans- retinol), making it available for LRAT processing to all-trans- retinyl ester.
Gene Information
| Human | |
|---|---|
| Gene Name: | RPE65 |
| Uniprot: | Q16518 |
| Entrez: | 6121 |
Family
| Belongs to: |
|---|
| carotenoid oxygenase family |
Alternative Names
All-trans-retinyl-palmitate hydrolase; EC 3.1.1.64; EC 5.2.1.7; LCA2p63; mRPE65; RBP-binding membrane protein; rd12; Retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific protein (65kD); retinal pigment epithelium-specific protein 65kDa; retinitis pigmentosa 20 (autosomal recessive); retinoid isomerohydrolase; Retinol isomerase; RP20; sRPE65
Molecular Weight
Mass (kDA):
60.948 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 1p31.3 |
| Sequence: | 1; NC_000001.11 (68428822..68450322, complement) |
Tissue Specificity
Retina (at protein level). Retinal pigment epithelium specific.
Subcellular Localizations
Cytoplasm. Cell membrane; Lipid-anchor. Microsome membrane. Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated. Undergoes light-dependent intracellular transport to become more concentrated in the central region of the retina pigment epithelium cells.
Diseases
- Leber Congenital Amaurosis
- Amaurosis
- Retinal Degeneration
- Blind Vision
- Retinal Diseases
- Retinal Dystrophies
- Dystrophy
- Retinitis Pigmentosa
- Age Related Macular Degeneration
- Optic Atrophy, Hereditary, Leber
- Visual Impairment
Pathways
- Regeneration
- Localization
- Phototransduction
- Cell Death
- Pathogenesis
- Transport
- Pigmentation
- Phagocytosis
- Aging
- Cell Differentiation
- Reflex
- Cell Proliferation
- Reverse Transcription
PTMs
- Palmitoylation
- Cleavage
- Phosphorylation
- Biotinylation
Research Areas
- Cellular Markers
- Lipid and Metabolism
- Neuronal Cell Markers
- Neuroscience
- Sensory Systems
- Signal Transduction
- Vision
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RPE65
bosterbio.com/bosterbio-gene-info-cards/RPE65
References
PMID: 7633413 by Nicoletti A., et al. Molecular characterization of the human gene encoding an abundant 61 kDa protein specific to the retinal pigment epithelium.
PMID: 9501220 by Morimura H., et al. Mutations in the RPE65 gene in patients with autosomal recessive retinitis pigmentosa or Leber congenital amaurosis.