This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Regulatory-associated protein of mTOR.
Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which contributes to the activation of this RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling into mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complicated and the Rag GTPases.
Human | |
---|---|
Gene Name: | RPTOR |
Uniprot: | Q8N122 |
Entrez: | 57521 |
Belongs to: |
---|
WD repeat RAPTOR family |
KIAA1303; KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats; KOG1; Mip1; p150 target of rapamycin (TOR)-scaffold protein; Raptor; regulatory associated protein of MTOR, complex 1; regulatory-associated protein of mTOR; RPTOR
Mass (kDA):
149.038 kDA
Human | |
---|---|
Location: | 17q25.3 |
Sequence: | 17; NC_000017.11 (80544838..80966368) |
Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.
Cytoplasm. Lysosome. Cytoplasmic granule. Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.
We'll be discussing the benefits of a boster antibody and the benefits of using it in your research. Our antibodies are extremely specific and made in GMP facilities. They are frequently cited in the research community. They are reliable and efficient. Moreover, Boster antibodies have been validated for ELISA, Western Blotting, and Immunohistochemistry.
If you're a researcher, you're likely to trust the primary antibodies offered by Boster Bio. Their products are renowned for their high-affinity, proven performance in Western Blotting, Immunohistochemistry, and ELISA. Boster products are also made in-house to ensure high quality, efficiency, and consistency. Boster's antibodies also available for mouse, rabbit, or human samples
CiteAb data, which is derived from thousands upon thousands of antibody references, gives you a quantitative view of the research antibody marketplace. CiteAb data has scientific journals backing it. It reflects reliability and reproducibility. CiteAb makes it easy to determine which antibodies are most reproducible. This information can help you make informed purchases for your next research antibodies. Boster's quality-assured antibodies will help you make an informed decision.
As an added bonus, Boster's primary antibodies have high affinity and specificity. These antibodies can be used for a variety if research topics and applications such as cell biology and immunohistochemistry. While primary antibodies are generally unconjugated, they can be conjugated based on the assay requirements. Conjugated antibodies can be used in research without the need for secondary antibodies. These antibodies are also available as conjugated products so you can be sure they're both reliable.
Boster, another quality-assured firm, produces high-quality monoclonal as well as polyclonal antibodies that are used in research. Their primary antibodies are an asset to research in the fields of agriculture and plant biology. They are high quality tools that speed research and are backed with a 100% customer satisfaction guarantee. They are the preferred choice for researchers. The company also produces and licenses antibody for thousands of target animals.
Using genetically modified cells as a control for the primary antibody is an effective method for primary antibody quality control. These antibodies are made using an animal model lacking a specific gene involved in the expression of the antigen. The tissue taken from knockout animals can then be fixed and prepared in the exact same way as the original sample. Since the primary antibody is unable to bind the tissue, the tissue from knockout animals acts as a control.
Primary antibodies are raised against a particular antigen. Boster's antibodies are highly specific against the RPTOR marker, allowing researchers to study the protein's structure, post-translational modifications, and molecular interactions in living cells. Multiple research applications and validations in diverse species have proven the antibody's specificity. The antibodies are validated by Western Blotting, Immunohistochemistry, and ELISA.
Absorption controls are useful for verifying that the antibody is specifically against one protein. To rule out the possibility that the primary antibodies may bind to multiple antigens, however, an absorption control must also be used. Boster's primary antibodies are very specific for the RPTOR markers to prevent this. However, they can still contain contaminants and cannot be used in immunoblotting.
For immunocytochemistry, the RPTOR marker can be detected using a fluorescent secondary antibody or an immunocytochemistry-labeled cell line. GFP is used in immunocytochemistry to label the primary antibody. Cells are then stained using a second primary antibodies against the same antigen. The secondary antibody can also detect the primary antibody.
The clonality of primary antibodies is determined by the type of B cells used to produce the antibody. Monoclonal antibodies are produced by identical cells, while monoclonal ones are made by one cell. Monoclonal antibodies are more precise due to the antigen/epitope purification process. Polyclonal antibodies, on the other hand, are more robust and tolerant of minor changes in the antigen.
Molecular immunocytochemistry provides many benefits. For example, conventional mIHC relies on antibodies raised in a different species and isotype. Although fluorescent mIHC detects multiple markers, it is limited to only three markers by the available filter sets. This method requires a unique primary antibody for each species. This allows for the use of different species and isotypes in order to test various proteins.
Boster Bio offers over 16,000 antibodies that have been tested for quality. Primary antibodies are vital tools for many research projects. Boster antibodies have been validated for ELISA (WB), FC (FC), and IHC applications. Among the Boster products are rabbit polyclonal antibodies. Customers can get a complimentary secondary antibody with the purchase or primary antibodies. The Boster Quality Guarantee guarantees that all Boster antibodies will work as advertised.
Biofunctional antibodies mimic the biological effects ligand-receptor interactions. They can directly impact the cell or molecule being investigated. Biofunctional antibodies can be used in in vitro and in-vivo research. They are manufactured in a GMP facility. GMP manufacturing procedures ensure that the company's products meet clinical and research requirements. These antibodies have been manufactured in a dedicated GMP manufacturing facility that complies with MDSAP 2016 and ISO 13485 2016.
Boster's antibodies are produced in a GMP laboratory. Moreover, they are produced under the strictest quality control guidelines to ensure quality. All Boster primary antibodies are produced according to GMP guidelines and are therefore safe to use in human studies. If you are looking for high-quality antibodies for research, consider Boster Bio's Super Vision Detection kits. The innovative detection systems allow researchers to identify proteins of particular interest with greater sensitivity.
Primary antibodies are made by using immunogenic substances called immunogens. These substances can be full length proteins, protein fragments, or peptides. These substances may be whole organisms, cells or tissues. A datasheet lists the immunogens used in polyclonal primaries antibodies. To be effective, these antibodies need to match a specific region on a target proteins. To detect a cell's surface protein, the extracellular domain is used.
Boster places importance on the needs and goals of its customers. They strive to remain at the forefront and provide high-quality, innovative products. Boster has high-quality standards for its antibodies and ELISA kits. Each product is monitored closely to ensure that they meet the highest quality standards. There is no reason not to be high-quality in your research.
Boster's products as primary antibodies are made under strict GMP regulations. Boster's GMP-certified facility ensures consistent quality. Boster has put in place strict procedures to ensure their products are of high quality. To ensure safety in research and clinical studies, the primary antibodies are checked for quality and consistency. Boster can help if you have questions!
PMID: 12150925 by Kim D.-H., et al. mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the growth machinery.
PMID: 12150926 by Hara K., et al. Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action.