Click image to see more details
Product Info Summary
| SKU: | M01463 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Raptor RPTOR Rabbit Monoclonal Antibody
SKU/Catalog Number
M01463
BM4438 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Raptor RPTOR Rabbit Monoclonal Antibody catalog # M01463. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Raptor RPTOR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01463)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EGB-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Raptor
Reactive Species
M01463 is reactive to RPTOR in Human, Mouse, Rat
Observed Molecular Weight
149 kDa
Calculated molecular weight
149.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01463 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
FC 1:30
Positive Control
WB: MCF-7 cell
Validation Images & Assay Conditions
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Western blot analysis of Raptor expression in MCF-7 cell lysate.
Specific Publications For Anti-Raptor RPTOR Rabbit Monoclonal Antibody (M01463)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Raptor RPTOR Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Raptor Rabbit Monoclonal antibody (M01463)?
Verified Customer
Verified customer
Asked: 2019-03-05
Answer
We do provide the blocking peptide for product anti-Raptor Rabbit Monoclonal antibody (M01463). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-03-05
Question
Does anti-Raptor Rabbit Monoclonal antibody M01463 work on horse IP with brain?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
Our lab technicians have not tested anti-Raptor Rabbit Monoclonal antibody M01463 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Raptor Rabbit Monoclonal antibody M01463 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-14
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Raptor Rabbit Monoclonal antibody M01463. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-24
Question
Would anti-Raptor Rabbit Monoclonal antibody M01463 work for IP with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
According to the expression profile of leukemic t-cell, RPTOR is highly expressed in leukemic t-cell. So, it is likely that anti-Raptor Rabbit Monoclonal antibody M01463 will work for IP with leukemic t-cell.
Boster Scientific Support
Answered: 2017-11-07
Question
We are currently using anti-Raptor Rabbit Monoclonal antibody M01463 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
K. Mangal
Verified customer
Asked: 2016-12-14
Answer
The anti-Raptor Rabbit Monoclonal antibody (M01463) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-14
