Anti-Raptor Rptor Antibody

Product Name

Anti-Raptor Rptor Antibody

View all RPTOR Antibodies

SKU/Catalog Number

A01463

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Raptor Rptor Antibody (Catalog # A01463). Tested in ELISA, WB, ICC applications. This antibody reacts with Mouse.

Storage & Handling

Raptor antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Raptor Rptor Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01463)

Host

Rabbit

Contents

Raptor Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Raptor (CT) polyclonal antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of mouse Raptor. The immunogen is located within amino acids 1140 - 1190 of Raptor.

Cross-reactivity

Raptor has multiple isoforms that may also be recognized by antibody.

Reactive Species

A01463 is reactive to RPTOR in Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

149.5 kDa

Background of RPTOR

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01463 is guaranteed for ELISA, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Raptor antibody can be used for the detection of Raptor by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information RPTOR

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