RFC4 Antibodies

AND RFC4 ELISA kits, RFC4 Proteins

Many biological assays use antibodies to detect RFC4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RFC4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RFC4 antibodies...

We validate the specificity of these antibodies to RFC4 by testing them on tissues known to express RFC4 positively and negatively. Browse below to find the RFC4 antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for RFC4? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the RFC4 Western Blot Guide

RFC4 Infographic by Boster Bio

All RFC4 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-RFC4 Antibody Picoband®

Western blot analysis of RFC4 using anti-RFC4 antibody (A07702-1)

IF analysis of RFC4 using anti-RFC4 antibody (A07702-1) and anti-Beta Tubulin antibody (M01857-3)

Flow Cytometry analysis of Raji cells using anti-RFC4 antibody (A07702-1)

Human

ELISA, Flow Cytometry, IF, ICC, WB

$370
Cat # A07702-1
100 µg/vial

RFC4 (NM_002916) Human Over-expression Lysate

RFC4 Human Over-expression Lysates NM_002916

Human

$628
Cat # LS005984
20 µg, 100 µg

RFC4 Overview

Facts about Replication factor C subunit 4.

RFC4 3D structure. Source: alphafold

Replication factor C subunit 4 (RFC4)

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.

Gene Information

Human
Gene Name:	RFC4
Uniprot:	P35249
Entrez:	5984

Family

Belongs to:

activator 1 small subunits family

Alternative Names

A1 37 kDa subunit; A1; Activator 1 37 kDa subunit; Activator 1 subunit 4; MGC27291; replication factor C (activator 1) 4, 37kDa; Replication factor C 37 kDa subunit; replication factor C subunit 4; RFC 37 kDa subunit; RF-C 37 kDa subunit; RFC37replication factor C (activator 1) 4 (37kD)

Molecular Weight

Mass (kDA):
39.682 kDA

Genomic Context


Human

Location:	3q27.3
Sequence:	3; NC_000003.12 (186789892..186806695, complement)

Subcellular Localizations

Nucleus.

Diseases

Carcinoma
Malignant Neoplasms
Neoplasms
Liver Carcinoma
Neoplasm Metastasis
Head And Neck Carcinoma
Neoplasm Invasiveness
Malignant Neoplasm Of Lung
Malignant Tumor Of Cervix
Head And Neck Neoplasms
Malignant Squamous Cell Neoplasm
Non-small Cell Lung Carcinoma
Tissue Adhesions

Liver Neoplasms
Cholangiocarcinoma
Metastatic Malignant Neoplasm To The Lymph Node
Malignant Neoplasm Of Breast
Uterine Cervical Neoplasm
Hepatitis B
Carcinogenesis

Interacting Proteins

RFC2
RFC3
RFC5
RNF41

Pathways

Dna Replication
Cell Cycle
Dna Repair
Dna Damage Checkpoint
S Phase
Cell Growth
Transport
Cell Adhesion
Sister Chromatid Cohesion
Cell Proliferation
Single-stranded Dna Binding
Cell Cycle Checkpoint
Proteolysis

Immune Response
Chromosome Segregation
Cell Division
Fermentation
Translesion Synthesis
Dna Modification
Telomere Maintenance

PTMs

Phosphorylation

Research Areas

Cell Cycle and Replication
DNA Repair

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RFC4

References

PMID: 1351677 by Chen M., et al. Studies of the cloned 37-kDa subunit of activator 1 (replication factor C) of HeLa cells.

PMID: 11572977 by Lindsey-Boltz L.A., et al. Purification and characterization of human DNA damage checkpoint Rad complexes.