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- Table of Contents
SERPINI1 · Western blot design guide
SERPINI1 Western Blot Planning Guide
Plan a SERPINI1 Western blot around the catalog-observed 46.4 kDa band, image-backed PB9744 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
SERPINI1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 46.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Cerebral cortex | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 46.4 kDa |
| ⓘ | Localization | Secreted / Cytoplasmic vesicle, secretory vesicle lumen |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated SERPINI1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Cerebral cortex |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9744 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected SERPINI1 Western Blot Band Size?
Use the product-observed 46.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 46.4 kDa | Matches the authoritative product WB observation. |
| 46.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected SERPINI1 appearancePlan around 46.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 46.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 46.4 kDa from UniProt Q99574; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9744. |
| Situation | Likely cause | Next action |
|---|---|---|
| 46.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing SERPINI1 band
PB9744 · SERPINI1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SERPINI1 Western blot
🧪Use Cerebral cortex as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Cerebral cortex (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for SERPINI1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebral cortex | Reported tissue cells | High | Protein (HPA) | HPA → |
| Hippocampus | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Placenta | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 3
Advanced SERPINI1 Western Blot Tips
Deeper troubleshooting and optimisation questions for SERPINI1, answered from its protein features.
Which band should guide the blot?
Use 46.4 kDa, the observation attached to the authoritative PB9744 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 46.4 kDa expectation.
Which positive control should I start with?
Start with Cerebral cortex, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for SERPINI1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9744 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
SERPINI1 Western Blot Reagents
Human/Mouse/Rat-reactive SERPINI1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-Neuroserpin/SERPINI1 Antibody Picoband®
Cat # PB9744
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q99574
- Human Protein Atlas — SERPINI1
- PB9744 product record
- PMC11908049 — SERPINI1 serves as a biomarker promoting cell proliferation and invasion in hepatocellular carcinoma (Cancer cell international, 2025)
- PMC10975625 — Quantitative Proteomics Reveal Region-Specific Alterations in Neuroserpin-Deficient Mouse Brain and Retina: Insights into Serpini1 Function (Proteomes, 2024)
- PMC3360813 — MicroRNA-21 inhibits Serpini1, a gene with novel tumour suppressive effects in gastric cancer (Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 2012)