SLC22A7 Antibodies

Solute carrier family 22 member 7 (SLC22A7)

Mediates sodium-independent multispecific organic anion transport. Transport of prostaglandin E2, prostaglandin F2, tetracycline, bumetanide, estrone sulfate, glutarate, dehydroepiandrosterone sulfate, allopurinol, 5-fluorouracil, paclitaxel, L-ascorbic acid, salicylate, ethotrexate, and alpha- ketoglutarate.

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Gene Information

Human
Gene Name:	SLC22A7
Uniprot:	Q9Y694
Entrez:	10864

Family

Belongs to:
major facilitator (TC 2.A.1) superfamily

Alternative Names

hOAT2; liver-specific transporter; NLTMGC45202; Novel liver transporter; OAT2MGC24091; Organic anion transporter 2; solute carrier family 22 (organic anion transporter), member 7; solute carrier family 22 member 7

Molecular Weight

Mass (kDA):
60.026 kDA

Genomic Context

Human
Location:	6p21.1
Sequence:	6; NC_000006.12 (43295710..43305538)

Tissue Specificity

Expressed in liver and kidney.

Subcellular Localizations

Basolateral cell membrane; Multi-pass membrane protein. Apical side of the renal tubule.

Diseases

• Malignant Neoplasms
• Malignant Neoplasm Of Breast
• Neoplasms
• Hyperuricemia
• Carcinoma
• Hyperbilirubinemia
• Cholestasis
• Rhinorrhea
• Liver Diseases
• Drug-induced Liver Injury
• Multiple Organ Failure
• Osteosclerosis

• Liver Damage
• Injury To Liver
• Icterus
• Mammary Neoplasms
• Hay Fever
• Dysequilibrium Syndrome

Pathways

• Transport
• Excretion
• Localization
• Secretion
• Drug Transport
• Anion Transport
• Organic Anion Transport
• Righting Reflex
• Cell Proliferation
• Organic Cation Transport
• Methotrexate Transport
• Cation Transport

• Sulfate Transport
• Liver Development
• Reflex
• Transepithelial Transport
• Urea Cycle
• Reverse Transcription
• Antiport

PTMs

• Carboxylation

• Hydroxylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SLC22A7

Boster Bio Anti-Glycophorin A / CD235a (Erythrocyte Marker) Monoclonal Antibody

The Boster Bio Anti–Glycophorin/CD235a Monoclonal Antibody is a good choice if you're looking for a monoclonal antibody. While it might seem like a bizarre option however, it's actually a good option for a majority of scientists. The product doesn't only detect the human embryo's protein but also detects the SLC22A7 protein within these cells. It can be used in research and other applications. Researchers from all over the globe have product credits.

Boster Bio Anti-GlycophorinA / CD235a (Erythrocyte Marker) Monoclonal Antibody

The Boster Bio Anti-GlycophorinA / CD235a (Erythrocytosis) MonoclonalAntibody was developed using GYPA as the antigen. It is approved for use in Immunohistochemistry, WB. Its high specificity makes it suitable for a range of applications in immunochemistry.

Researchers across the globe can utilize the product for research and development. The sensitivity of the antibody was tested in ELISA and by immunohistochemistry using a panel of more than 250 samples. It is also suitable for use in flow cytometry, immunohistochemistry, and immunofluorescence. Boster Bio has validated the ELISA kit using recombinant protein and non-transfected cells.

The monoclonal antibody against glycophorin A/CD235a recognizes the cytoplasmic area of human erythrocytes and erythroid precursor cells. Its phosphorylated surface can reduce the aggregation of red blood cells. Anti-GlycophorinA/CD235a monoclonal antibodies react to GlycophorinA in the cellular membranes of mature erythrocytes and erythroid precursors.

The HIR2 antibody was examined in normal human peripheral blood cells. Results showed that HIR2 causes cells to become agglutinated once the antibodies are higher in concentration. The recommended concentration for antibodies is 0.03 mg per test. It is best to determine the amount of cells required for each test. It could range between 105 and 110 cells/test. The HIR2 antibody should be adjusted properly to ensure optimal performance.

ARP American Research Products, Inc. (ARP), disclaims all warranties and is not responsible for the compliance with applicable laws or intellectual rights permissions. ARP and its affiliates do not assume responsibility for any outcomes resulting from the use of its products. Boster Bio will not refund any research that was not conducted or used in a way that is not appropriate.

K562 cells were transfected with recombinant lentiviral particles with shRNA against ZNF268. Transfected cells were then sorted according to GFP expression and cell proliferation. K562 cells were exposed to lentiviral particles expressing ZNF268 RNA to assess the effectiveness of this monoclonal antibody.

Cell count, proliferation assay in immunohistochemistry and cell count were all conducted. The cells were cultivated in 24-well plates, with 1x105 cells per well. Every every other day, half the media was replaced and the proliferation assessed. The proliferation was also evaluated using the EdU incorporation test. Cells in culture were exposed to 50 uM of EdU for 4 hours at 37°C. Following this the cells were fixed in 4 percent formaldehyde over 30 min at room temperature and then permeated with 0.5 percent Triton X100 and incubated with 400 ul 1XApollo(r) reaction mix for 30 min.

Scientists developed the monoclonal antigen against Glycophorin/CD235a (Erythrocyte Mark). The antibody is able to detect blood cells, including peripheral blood lymphocytes. The results were obtained after transiently the transfection of HEK293 and HeLa cells with Lipofectamine 2000. The resulting cDNA was used for real-time quantitative PCR using the ABI 7500 system. GAPDH, another marker for the human immune system, was also used as a standard.

The human ZNF268 gene encodes a common KRAB-C2H2 zinc-finger protein. It is believed that the gene plays a role in hematopoiesis and leukemogenesis. ZNF268 expression is decreased in erythropoiesis. GATA-1 is also believed to inhibit transcription and promoter activity for ZNF268. Multiple evidence-based lines confirm its role in leukemia and hematopoiesis. Moreover, Krackhardt et al. Krackhardt and al.

The monoclonal antibody has been tested using K562 cells. It was washed twice with phosphate-buffered saline. The precipitated DNA was then amplified by PCR and stained with ethidium bromide. Ethidium bromide staining was employed to reveal the products of PCR amplification.

GATA-1 binds to its binding site located in the promoter of the gene. Wild-type G1 probes that are not labeled compete with biotin-labeled probes by 200-fold. The inactive mutant GATA-1 did not interfere with GATA-1 binding, and the anti-GATA-1 antibody was not hindered by the presence FOG.

Monoclonal antibody detects a protein known as Glycophorin A. It is located on blood vessels' surface and is essential for proper functioning of the immune system. It is a protein that is found in red blood cells. Also known as erythrocytes is also believed to be expressed in these cells. The anti-Glycophorin A monoclonal antibodies are available in a variety of formats.

The Boster Bio Anti-Glycophoriin A CD235a (Eerythrocyte Marker) Monoclonabody was designed for analyzing hematopoietic stem cells and suppressing the differentiation of the erythroid precursors. This antibody is a powerful marker of erythroids that can be utilized as an effective therapeutic tool.

The Boster Bio Anti-Glycophoria A/CD235a (Erythrocyte Markers) Monoclonal Antibody is a unique protein that is able to bind to the ZNF268 promoter. It is designed to recognize human erythrocytes by binding to its GATA-binding site.