VPS28 Antibodies

AND VPS28 ELISA kits, VPS28 Proteins

Many biological assays use antibodies to detect VPS28, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with VPS28 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop VPS28 antibodies...

We validate the specificity of these antibodies to VPS28 by testing them on tissues known to express VPS28 positively and negatively. Browse below to find the VPS28 antibody that suites your experiment. We have 6 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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VPS28 Infographic by Boster Bio

All VPS28 Products

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Applications

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Anti-VPS28 Antibody

Western blot analysis of extracts of various cell lines, using VPS28 antibody at 1:1000 dilution

Human, Mouse, Rat

IF, ICC, WB

$405
Cat # A06407
100ul

VPS28 (NM_183057) Human Recombinant Protein

Coomassie blue staining of purified VPS28 protein

$1249
Cat # PROTQ9UK41
20 µg

Anti-VPS28 Mouse Monoclonal Antibody [Clone ID: OTI1A8]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VPS28 (Right lane) cDNA for 48 hrs and lysed

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-VPS28 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-VPS28 monoclonal antibody (1:200)

Dog, Human, Monkey, Mouse, Rat

IF, IHC, WB

$523
Cat # M06407
100 µl

Anti-VPS28 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature

Immunofluorescent analysis using the Antibody at 1:150 dilution

Human, Mouse, Rat

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # M06407-1
100 μl/vial

VPS28 (NM_016208) Human Over-expression Lysate

VPS28 Human Over-expression Lysates NM_016208

Human

$628
Cat # LS051160
20 µg, 100 µg

Anti-VPS28 Antibody

Human, Mouse, Rat

ELISA, IP, IF, ICC, WB

$370
Cat # A06407-2
100 μl/vial

VPS28 Overview

Facts about Vacuolar protein sorting-associated protein 28 homolog.

VPS28 3D structure. Source: alphafold

Vacuolar protein sorting-associated protein 28 homolog (VPS28)

Part of the ESCRT-I complicated, a regulator of vesicular trafficking process. .

Gene Information

Human
Gene Name:	VPS28
Uniprot:	Q9UK41
Entrez:	51160

Family

Belongs to:

VPS28 family

Alternative Names

ESCRT-I complex subunit VPS28; H-Vps28; MGC60323; vacuolar protein sorting 28 (yeast); vacuolar protein sorting 28 homolog (S. cerevisiae); vacuolar protein sorting-associated protein 28 homolog; yeast class E protein Vps28p homolog

Molecular Weight

Mass (kDA):
25.425 kDA

Genomic Context


Human

Location:	8q24.3
Sequence:	8; NC_000008.11 (144423601..144428563, complement)

Subcellular Localizations

Cell membrane. Late endosome membrane; Peripheral membrane protein.

Diseases

Hiv Infections
Immunologic Deficiency Syndromes
Neoplasms
Leukemia
Anemia
Equine Infectious Anemia
Candidiasis
Influenza
Malnutrition
Liver Carcinoma
Myeloid Leukemia, Chronic

Carcinoma
Myeloid Leukemia
Headache
Vertical Disease Transmission
Aortic Aneurysm, Abdominal

Interacting Proteins

BLZF1
GOLGA2
SAXO1
SKIL
SMN1

TRIM27
TSG101
TSNAX
USHBP1
VPS37B

VPS52

Pathways

Transport
Cytokinesis
Virulence
Actin Cytoskeleton Organization
Sperm Individualization
Response To Ph
Ubiquitin Homeostasis
Hypersensitivity
Endocytosis
Localization
Methylation

Cytoskeleton Organization
Cellularization
Membrane Invagination
Lipid Binding
Vacuolar Transport
Protein Processing

PTMs

Ubiquitination

Phosphorylation

Research Areas

Autophagy

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/VPS28

References

PMID: 11916981 by Bishop N., et al. Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates.

PMID: 14505570 by von Schwedler U.K., et al. The protein network of HIV budding.