VRK1 Antibodies

AND VRK1 ELISA kits, VRK1 Proteins

Many biological assays use antibodies to detect VRK1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with VRK1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop VRK1 antibodies...

We validate the specificity of these antibodies to VRK1 by testing them on tissues known to express VRK1 positively and negatively. Browse below to find the VRK1 antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for VRK1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the VRK1 Western Blot Guide

VRK1 Infographic by Boster Bio

All VRK1 Products

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Product

Figures

Specificity

Applications

Price

Anti-VRK1 Antibody Picoband®

Western blot analysis of VRK1 using anti-VRK1 antibody (PB9907)

Human, Rat

$370
Cat # PB9907
100 μg/vial

VRK1 (NM_003384) Human Over-expression Lysate

Human

$628
Cat # LS007443
20 µg, 100 µg

VRK1 Overview

Facts about Serine/threonine-protein kinase VRK1.

VRK1 3D structure. Source: alphafold

Serine/threonine-protein kinase VRK1 (VRK1)

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: after phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein.

Phosphorylates'Thr- 18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3.

Gene Information

Human
Gene Name:	VRK1
Uniprot:	Q99986
Entrez:	7443

Family

Belongs to:

protein kinase superfamily

Alternative Names

EC 2.7.11.1; MGC117401; MGC138280; MGC142070; PCH1; serine/threonine-protein kinase VRK1; vaccinia related kinase 1; vaccinia virus B1R-related kinase 1; Vaccinia-related kinase 1; VRK1

Molecular Weight

Mass (kDA):
45.476 kDA

Genomic Context


Human

Location:	14q32.2
Sequence:	14; NC_000014.9 (96797382..96881614)

Tissue Specificity

Widely expressed. Highly expressed in fetal liver, testis and thymus.

Subcellular Localizations

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.

Diseases

Neoplasms
Vaccinia
Malignant Neoplasms
Atrophy
Muscular Atrophy
Female Infertility
Leukemia
Malignant Neoplasm Of Breast
Infertility
Carcinoma
Chromosome Breakage
Mammary Neoplasms
Spinal Muscular Atrophy

Precursor T-cell Lymphoblastic Leukemia-lymphoma
Sterility
Muscle Weakness
Weakness
Adult T-cell Lymphoma/leukemia
Lung Neoplasms
Male Infertility

Interacting Proteins

ATF2
AURKB
COIL
H2AFX
JUN

NBN
PLK3
RAN
RNF8
SOX2

TP53
TP53BP1

Pathways

Cell Cycle
Cell Proliferation
Localization
Cell Division
Mitosis
Dna Replication
Chromosome Segregation
Cell Cycle Arrest
Histone Phosphorylation
Meiosis
Transport
Protein Phosphorylation
Hyperphosphorylation

Cell Cycle Phase
Nuclear Envelope Disassembly
Translation
Female Meiosis
Telomere Maintenance
Chromatin Organization
Interphase

PTMs

Phosphorylation
Acetylation

Dephosphorylation
Ubiquitination

Reduction

Research Areas

Core ESC-Like Genes
Protein Kinase
Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/VRK1

References

PMID: 9344656 by Nezu J., et al. Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to Vaccinia virus B1R kinase.

PMID: 10951572 by Lopez-Borges S., et al. The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine- 18 within the mdm-2 binding site of the p53 tumour suppressor protein.