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- Table of Contents
Wee1-like protein kinase Antibodies
AND WEE1 ELISA kits, Wee1-like protein kinase Proteins
Many biological assays use antibodies to detect Wee1-like protein kinase, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Wee1-like protein kinase in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Wee1-like protein kinase antibodies...
Designing a Western blot for Wee1-like protein kinase? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the WEE1 Western Blot GuideDesigning an IHC experiment for Wee1-like protein kinase? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the WEE1 IHC GuideWEE1 Infographic by Boster Bio
All WEE1 Products
Product
Figures
Specificity
Applications
Price
Anti-CPS1 Antibody Picoband®
2 Citations
Human, Monkey, Mouse, Rat
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A01320-2
100 μg/vial
Cat # A01320-2
100 μg/vial
Human, Rat
ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB
$370
Cat # A01325-3
100 µg/vial
Cat # A01325-3
100 µg/vial
Human, Monkey
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A01326-1
100 μg/vial
Cat # A01326-1
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A01319-1
100 μg/vial
Cat # A01319-1
100 μg/vial
Human, Mouse, Rat
ELISA
$540
Cat # EKC2330
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2330
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$480
Cat # EKC1599
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1599
1 kit, containing one 96-well plate and all necessary reagents
WEE1 Overview
Facts about Wee1-like protein kinase.
Wee1-like protein kinase (WEE1)
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase.
Phosphorylation of cyclin B1-CDK1 happens only on'Tyr-15' and phosphorylation of monomeric CDK1 doesn't occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated.
Gene Information
| Human | |
|---|---|
| Gene Name: | WEE1 |
| Uniprot: | P30291 |
| Entrez: | 7465 |
Family
| Belongs to: |
|---|
| protein kinase superfamily |
Alternative Names
DKFZp686I18166; EC 2.7.10.2; FLJ16446; WEE1 homolog (S. pombe); wee1+ (S. pombe) homolog; WEE1+ homolog; Wee1A kinase; WEE1A; WEE1hu; wee1-like protein kinase
Molecular Weight
Mass (kDA):
71.597 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 11p15.4 |
| Sequence: | 11; NC_000011.10 (9573670..9589985) |
Subcellular Localizations
Nucleus.
Diseases
- Malignant Neoplasms
- Neoplasms
- Carcinoma
- Encephalomyelitis, Western Equine
- Leukemia
- Cell Transformation, Neoplastic
- Malignant Neoplasm Of Breast
- Mammary Neoplasms
- Growth Arrest
- Endoreduplication
- Colonic Neoplasms
- Malignant Tumor Of Colon
Interacting Proteins
- BTRC
- FBXW11
- YWHAZ
Pathways
- Cell Cycle
- Mitosis
- Cell Division
- Cell Cycle Arrest
- Dna Replication
- S Phase
- Interphase
- Cell Death
- M Phase
- Cell Proliferation
- Localization
- Cell Growth
- Dna Damage Checkpoint
PTMs
- Phosphorylation
- Dephosphorylation
- Cleavage
Research Areas
- Cell Cycle and Replication
- Checkpoint signaling
- Core ESC-Like Genes
- DNA Double Strand Break Repair
- DNA Repair
- Stem Cell Markers
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/WEE1
bosterbio.com/bosterbio-gene-info-cards/WEE1
References
PMID: 7743995 by Watanabe N., et al. Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle.
PMID: 11528126 by Cichutek A., et al. Comparative architectural aspects of regions of conserved synteny on human chromosome 11p15.3 and mouse chromosome 7 (including genes WEE1 and LMO1).
1 Publications of Boster Wee1-like protein kinase Antibodies & ELISA Kits
- PubMed ID: 10.1261/rna.041822.113, The Streptococcus suis transcriptional landscape reveals adaptation mechanisms i...
*More publications can be found for each product on its corresponding product page