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- Table of Contents
XDH · Western blot design guide
XDH Western Blot Planning Guide
Plan a XDH Western blot around the catalog-observed 146.4 kDa band, image-backed A01884 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
XDH Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 146.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Lactating breast | |
| Negative control | Adrenal gland |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 146.4 kDa |
| ⓘ | Localization | Cytoplasm / Peroxisome |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated XDH Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Lactating breast |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01884 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected XDH Western Blot Band Size?
Use the product-observed 146.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 146.4 kDa | Matches the authoritative product WB observation. |
| 146.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected XDH appearancePlan around 146.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 146.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 146.4 kDa from UniProt P47989; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01884. |
| Situation | Likely cause | Next action |
|---|---|---|
| 146.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing XDH band
A01884 · XDH
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for XDH Western blot
🧪Use Lactating breast as the first positive-control candidate and Adrenal gland as the HPA Not detected negative candidate.
Positive control: Lactating breast (Medium)
Negative control: Adrenal gland (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for XDH
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Lactating breast | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Small intestine | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Kidney | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Duodenum | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 3
Advanced XDH Western Blot Tips
Deeper troubleshooting and optimisation questions for XDH, answered from its protein features.
Which band should guide the blot?
Use 146.4 kDa, the observation attached to the authoritative A01884 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 146.4 kDa expectation.
Which positive control should I start with?
Start with Lactating breast, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adrenal gland as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for XDH Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01884 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
XDH Western Blot Reagents
Human/Mouse/Rat-reactive XDH Western blot reagents with authoritative product imagery.
Real WB data
Anti-Xanthine Oxidase/XDH Antibody Picoband®
Cat # A01884
Real WB data
Anti-XDH Antibody Picoband®
Cat # A01884-2
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P47989
- Human Protein Atlas — XDH
- A01884 product record
- PMC9149634 — C1QBP regulates apoptosis of renal cell carcinoma via modulating xanthine dehydrogenase (XDH) mediated ROS generation (International journal of medical sciences, 2022)
- PMC6662189 — Xanthine oxidase-mediated oxidative stress promotes cancer cell-specific apoptosis (Free radical biology & medicine, 2019)
- PMC11526462 — [Molecular mechanism of high-altitude hypoxia-induced lipid metabolism disorder in mouse spleen tissue] (Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2024)
