Bovine Endothelin 1/EDN1 ELISA Kit PicoKine™

Bovine Endothelin 1/EDN1 ELISA Kit PicoKine™ (96 Tests). Quantitate Bovine EDN1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml.

Product Info Summary

SKU: EK0945-BV
Size: 96 wells/kit, with removable strips.
Reactive Species: Bovine
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Bovine Endothelin 1/EDN1 ELISA Kit PicoKine™

See all Endothelin-1 products

SKU/Catalog Number

EK0945-BV

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Bovine Endothelin 1/EDN1 ELISA Kit PicoKine™ (96 Tests). Quantitate Bovine EDN1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Bovine Endothelin 1/EDN1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0945-BV)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: bovine peptide; Immunogen sequence: C53-W73

Sensitivity

<0.5 pg/ml

Assay Range

3.9 pg/ml - 250 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0945-BV is reactive to EDN1 in Bovine samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK0945-BV is guaranteed for ELISA in Bovine by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0945-BV-CAP Anti-Bovine EDN1 Pre-coated 96-well strip microplate 1
EK0945-BV-ST Bovine EDN1 Standard 2 vials, 1 ng/tube
EK0945-BV-DA Bovine EDN1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03.97.815.631.262.5125250
O.D.0.0170.0450.0880.1570.3570.7721.6462.339

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Bovine Endothelin 1/EDN1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)26.185.414623.284.3143
Standard deviation1.413.768.031.796.078.72
CV (%)4.4%4.4%5.5%7.7%7.2%6.1%

Gene/Protein Information For EDN1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P05305

Gene ID

1906

Gene Name

EDN1

Full Name

Endothelin-1

Weight

24.425kDa

Superfamily

endothelin/sarafotoxin family

Alternative Names

ARCND3; EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME EDN1 ARCND3, ET1, HDLCQ7, PPET1, QME endothelin 1 endothelin-1|preproendothelin-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EDN1, check out the EDN1 Infographic

EDN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EDN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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16 Customer Q&As for Bovine Endothelin 1/EDN1 ELISA Kit PicoKine™

Question

Q: how can I thaw whole blood sample for Endothelin-1 ELISA after freezing?

J. Garcia

Verified customer

Asked: 2020-04-29

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Endothelin-1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-04-29

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-03-31

Question

How much concentration of sodium azide or thimerosal and 2-mercaptoethanol (or other toxic substances) is contained in EK0945-BV?

Verified customer

Asked: 2019-08-28

Answer

For the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV), Recombinant standard - sodium azide 0.02mg/vial Biotinylated antibody, sample, antibody, and abc diluent buffer - sodium azide 0.02%

Boster Scientific Support

Answered: 2019-08-29

Question

Does EK0945-BV contain protein derived from Baculovirus expression system?

Verified customer

Asked: 2019-08-28

Answer

For the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV), the expression system for standard is Bovine peptide.

Boster Scientific Support

Answered: 2019-08-29

Question

Does your shipping medium include animal origin components?

Verified customer

Asked: 2019-08-28

Answer

The shipping medium does not include animal origin components.

Boster Scientific Support

Answered: 2019-08-29

Question

What is the composition of the reaction stop solution for EK0945-BV?

Verified customer

Asked: 2019-08-28

Answer

The TMB stop solution for the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV) is Sulphuric acid - 2N.

Boster Scientific Support

Answered: 2019-08-29

Question

What does the abc diluent buffer for EK0945-BV contain?

Verified customer

Asked: 2019-08-27

Answer

ABC duiluent buffer for the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV) contains 0.02% sodium azide, 0.5% BSA, 0.2% Tris and 1% Nacl.

Boster Scientific Support

Answered: 2019-08-29

Question

Is the expression system for standard of EK0945-BV an unpurified peptide derived from cattle?

Verified customer

Asked: 2019-08-27

Answer

The immunogen of the standard for the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV) is synthesized peptide.

Boster Scientific Support

Answered: 2019-08-29

Question

Q: how to analyze ELISA data? I measured Endothelin-1 level in plasma.

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-05-15

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-01-11

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-01-11

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Endothelin-1 using the EK0945-BV Bovine Endothelin-1 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-11-18

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-11-18

Question

# A reagent containing sodium azide, but please let me know exactly what abc diluent buffer is. # Expression system for standard: Bovine peptide. Please let me know if this is an unpurified peptide derived from cattle. # stop solution: Sulfuric acid-2N The MSDS stated 2%. Please let me know which is correct, as the concentration is completely different between 2% and 2N.

Verified Customer

Verified customer

Asked: 2018-05-22

Answer

Please see the following answeres. # A reagent containing sodium azide, but please let me know exactly what abc diluent buffer is. ABC duiluent buffer contains 0.02% sodium azide, 0.5% BSA, 0.2% Tris and 1% Nacl. # Expression system for standard: Bovine peptide. Please let me know if this is an unpurified peptide derived from cattle. The immunogen of the standard is synthesized peptide. # stop solution: Sulfuric acid-2N The MSDS stated 2%. Please let me know which is correct, as the concentration is completely different between 2% and 2N. The stop solution is 2% sulfuric acid.

Boster Scientific Support

Answered: 2018-05-22

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-05-17

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-05-17

Question

Q: is it okay to use citrate plasma as samples in Bovine Endothelin-1 Picokine® ELISA Kit (Catalog # EK0945-BV)?

Verified Customer

Verified customer

Asked: 2018-03-16

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Endothelin-1 causing degradation of its protein structure. Endothelin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Endothelin-1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-03-16

Question

Q: What is the optimal O.D. value for Endothelin-1 ELISA kit? I performed your Endothelin-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Endothelin-1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2017-09-21

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Endothelin-1 ELISA kit to have sensitivity of 0.5pg/ml, that means the minimum amount of Endothelin-1 that can be declared/interpreted as positive by the above standard is 0.5pg/ml.

Boster Scientific Support

Answered: 2017-09-21

Question

Q: if the enzyme conjugated Endothelin-1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Endothelin-1 antigen?

K. Martin

Verified customer

Asked: 2016-05-15

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-05-15

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