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Product Info Summary
| SKU: | EK2390 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Bovine |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum, plasma(heparin, EDTA or citrate). |
Product info
Product Name
Bovine IFN-gamma ELISA Kit PicoKine®
SKU/Catalog Number
EK2390
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Bovine IFN-gamma ELISA Kit PicoKine® (96 Tests). Quantitate Bovine IFN-gamma in cell culture supernatants, cell lysates, serum, plasma(heparin, EDTA or citrate). Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Bovine IFN-gamma ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2390)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: G24-M166
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<4 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2390 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2390 is reactive to IFNG in Bovine samples
Validated Sample Types
cell culture supernatants, cell lysates, serum, plasma(heparin, EDTA or citrate).
Application Guarantee
EK2390 is guaranteed for ELISA in Bovine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IFNG
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2390-CAP | Anti-Bovine IFNG Pre-coated 96-well strip microplate | 1 |
| EK2390-ST | Bovine IFNG Standard | 2 vials, 10 ng/tube |
| EK2390-DA | Bovine IFNG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.018 | 0.059 | 0.102 | 0.166 | 0.28 | 0.524 | 1.002 | 1.904 |
Data Example Images
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Bovine IL-2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Bovine IFN-gamma ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 21 | 80 | 475 | 17 | 80 | 250 |
| Standard deviation | 1.31 | 5.81 | 15.53 | 0.60 | 4.87 | 19.90 |
| CV (%) | 6.2% | 7.3% | 3.3% | 3.6% | 6.1% | 8.0% |
Specific Publications For Bovine IFN-gamma ELISA Kit PicoKine® (EK2390)
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