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Product Info Summary
| SKU: | EKC1069 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
C/EBP-alpha Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1069
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The C/EBP-alpha Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor C/EBP-alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of C/EBP-alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on C/EBP-alpha.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
C/EBP-alpha Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1069)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1069 is reactive to CEBPA in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1069 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-CEBPA Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from COS7 cells
Protein Target Info & Infographic
Gene/Protein Information For CEBPA (Source: Uniprot.Org, NCBI)
Gene Name
CEBPA
Full Name
CCAAT/enhancer-binding protein alpha
Weight
37.561kDa
Superfamily
bZIP family
Alternative Names
C/EBP alpha; C/EBP-alpha; CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer-binding protein alpha; CEBP alpha; CEBP; CEBPA CEBPA C/EBP-alpha, CEBP CCAAT enhancer binding protein alpha CCAAT/enhancer-binding protein alpha|CCAAT/enhancer binding protein (C/EBP), alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CEBPA, check out the CEBPA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CEBPA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For C/EBP-alpha Colorimetric Cell-Based ELISA Kit (EKC1069)
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for C/EBP-alpha Colorimetric Cell-Based ELISA Kit
Question
Hello, I am interested to purchase your EKC1069 C/EBP-alpha Colorimetric Cell-Based ELISA Kit. I noted that the immunofluorescence and flow cytometry are done in HeLa cells but not the Western blot. Can you provide a Western blot of HeLa cells using this EKC1069 C/EBP-alpha Colorimetric Cell-Based ELISA Kit? This is one of few cell types I'd be using the antibody in; I would like to see how clean it is. Thank you so much.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Currently we do not have an image of how EKC1069 C/EBP-alpha Colorimetric Cell-Based ELISA Kit works in western blot on HeLa cells. However, you can check all of the western blot data we have for this antibody on our website.
Boster Scientific Support
Answered: 2020-02-28
