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Product Info Summary
| SKU: | A00386S21 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-C/EBP Alpha (phospho-S21) CEBPA Antibody
SKU/Catalog Number
A00386S21
Size
100ul
Form
Liquid
Description
Boster Bio Anti-C/EBP Alpha (phospho-S21) CEBPA Antibody catalog # A00386S21. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C/EBP Alpha (phospho-S21) CEBPA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00386S21)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human C/EBP α around the phosphorylation site of Serine 21.
Cross-reactivity
Does not cross-react with eNOS or nNOS.
Reactive Species
A00386S21 is reactive to CEBPA in Human, Mouse, Rat
Calculated molecular weight
37.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00386S21 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of C/EBP α (phospho-S21) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with PBS(1×PBS,PH7.4) for 1 houre then treated with DMEM(10%FBS) for 5 minutes whole cell lysate Lane3:L02 treated with PBS(1×PBS,PH7.4) for 1 houre then treated with DMEM(10%FBS) for 10 minutes whole cell lysate Lane4:L02 treated with PBS(1×PBS,PH7.4) for 1 houre then treated with DMEM(10%FBS) for 15 minutes whole cell lysate
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Immunohistochemistry (IHC) analyzes of p-C/EBP α (S21) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.
Specific Publications For Anti-C/EBP Alpha (phospho-S21) CEBPA Antibody (A00386S21)
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