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Product Info Summary
| SKU: | EKC1077 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
CaMK1-alpha Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1077
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The CaMK1-alpha Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CaMK1-alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of CaMK1-alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CaMK1-alpha.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
CaMK1-alpha Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1077)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1077 is reactive to CAMK1 in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1077 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-CAMK1 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from COLO cells
Protein Target Info & Infographic
Gene/Protein Information For CAMK1 (Source: Uniprot.Org, NCBI)
Gene Name
CAMK1
Full Name
Calcium/calmodulin-dependent protein kinase type 1
Weight
41.337kDa
Superfamily
protein kinase superfamily
Alternative Names
calcium/calmodulin-dependent protein kinase I; calcium/calmodulin-dependent protein kinase type 1; CaM kinase I alpha; CaM Kinase I; CAMKI; caM-KI; caMKI-alpha; EC 2.7.11; EC 2.7.11.17; MGC120317; MGC120318 CAMK1 CAMKI calcium/calmodulin dependent protein kinase I calcium/calmodulin-dependent protein kinase type 1|caM kinase I alpha|caM-KI|caMKI-alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CAMK1, check out the CAMK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CAMK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CaMK1-alpha Colorimetric Cell-Based ELISA Kit (EKC1077)
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for CaMK1-alpha Colorimetric Cell-Based ELISA Kit
Question
Hi Support: Can you please specify the antigen used for EKC1077 CaMK1-alpha Colorimetric Cell-Based ELISA Kit. Is it N-terminal? C-terminal? or fusion protein of Calcium/Calmodulin-Dependent kinase 1 gamma?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
The immunogen for this EKC1077 CaMK1-alpha Colorimetric Cell-Based ELISA Kit is from the N terminus of Ca-Calmodulin kinase fused with GST.
Boster Scientific Support
Answered: 2020-02-27
