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Product Info Summary
| SKU: | P02576 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho CaMK1-alpha (Thr177) Antibody
SKU/Catalog Number
P02576
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho CaMK1-alpha (Thr177) Antibody (Catalog# P02576). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho CaMK1-alpha (Thr177) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02576)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177. Immunogen sequence location: 143-192
Reactive Species
P02576 is reactive to CAMK1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
41.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P02576 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK1-alpha (Phospho-Thr177) Antibody
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Immunofluorescence analysis of HeLa cells, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15' and Jurkat cells treated with insulin 0.01U/ml 15', using CaMK1-alpha (Phospho-Thr177) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho CaMK1-alpha (Thr177) Antibody (P02576)
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3 Customer Q&As for Anti-Phospho CaMK1-alpha (Thr177) Antibody
Question
Is this P02576 anti-Phospho CaMK1-alpha (Thr177) antibody reactive to the isotypes of CAMK1?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The immunogen of P02576 anti-Phospho CaMK1-alpha (Thr177) antibody is A peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177. Immunogen sequence location: 143-192. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-15
Question
I see that the anti-Phospho CaMK1-alpha (Thr177) antibody P02576 works with WB, what is the protocol used to produce the result images on the product page?
O. Krishna
Verified customer
Asked: 2019-02-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-02-08
Question
We are currently using anti-Phospho CaMK1-alpha (Thr177) antibody P02576 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
A. Krishna
Verified customer
Asked: 2017-01-18
Answer
The anti-Phospho CaMK1-alpha (Thr177) antibody (P02576) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-01-18
