Anti-PHAP ANP32A Antibody

Product Name

Anti-PHAP ANP32A Antibody

View all ANP32A Antibodies

SKU/Catalog Number

A03625-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PHAP ANP32A Antibody (Catalog # A03625-2). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PHAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PHAP ANP32A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03625-2)

Host

Rabbit

Contents

PHAP Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PHAP antibody was raised with a synthetic peptide corresponding to amino acids at amino terminus of human PHAP. The immunogen is located within the first 50 amino acids of PHAP.

Reactive Species

A03625-2 is reactive to ANP32A in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

28.6 kDa

Background of ANP32A

Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03625-2 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PHAP antibody can be used for detection of all three identified isoforms of PHAP including PHAP I, PHAP I2a, and PHAP III by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ANP32A

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