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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human S100A12/En Rage ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human S100A12/En Rage ELISA Kit PicoKine™ (96 Tests). Quantitate Human S100A12 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human S100A12/En Rage ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1169)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: M1-E92
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1169 is reactive to S100A12 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK1169 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1169-CAP||Anti-Human S100A12 Pre-coated 96-well strip microplate||1|
|EK1169-ST||Human S100A12 Standard||2 vials, 10 ng/tube|
|EK1169-DA||Human S100A12 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human S100A12 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect EN-RAGE/S100A12, Dilution ratio of 1:300, concentration in serum is around 80ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human S100A12/En Rage ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of EN-RAGE/S100A12 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For S100A12 (Source: Uniprot.Org, NCBI)
CAAF1; CAAF1Neutrophil S100 protein; CAAFI; CAGC; CAGCS100 calcium binding protein A12 (calgranulin C); CAGCS100; Calcium-binding protein in amniotic fluid 1; Calgranulin C; calgranulin-C; CGRPEN-RAGE; ENRAGE; EN-RAGE; Extracellular newly identified RAGE-binding protein; MRP6; p6calgranulin C; protein S100-A12; S100 calcium binding protein A12; S100 calcium-binding protein A12 (calgranulin C); S100 calcium-binding protein A12; S100A12 S100A12 CAAF1, CAGC, CGRP, ENRAGE, MRP-6, MRP6, p6 S100 calcium binding protein A12 protein S100-A12|EN-RAGE|calcitermin|calcium-binding protein in amniotic fluid 1|calgranulin C|extracellular newly identified RAGE-binding protein|migration inhibitory factor-related protein 6|neutrophil S100 protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on S100A12, check out the S100A12 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for S100A12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1169 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu T,Yin C,Li Y,Gao F,Yu L,Wang Z,Wang Q.Effects of Transcutaneous Electrical Acupoint Stimulation on Postoperative Cognitive Decline in Elderly Patients: A Pilot Study.Clin Interv Aging.2021 May 3;16:757-765.doi:10.2147/CIA.S309082.PMID:33976542;PMCID:PMC8106456.
EK1169 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
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