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Product Info Summary
| SKU: | LS001033 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of cyclin-dependent kinase inhibitor 3 (CDKN3), transcript variant 1 |
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Product info
Product Name
CDKN3 (NM_005192) Human Over-expression Lysate
SKU/Catalog Number
LS001033
Size
100 µg
Description
Transient overexpression lysate of cyclin-dependent kinase inhibitor 3 (CDKN3), transcript variant 1
Storage & Handling
Cite This Product
CDKN3 (NM_005192) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001033)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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CDKN3 Human Over-expression Lysates NM_001130851.
Western blot validation of overexpression lysate (LS001033) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CDKN3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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CDKN3 Human Over-expression Lysates NM_005192.
Western blot validation of overexpression lysate (LS001033) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CDKN3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CDKN3 (Source: Uniprot.org, NCBI)
Gene Name
CDKN3
Full Name
Cyclin-dependent kinase inhibitor 3
Weight
23.805kDa
Superfamily
protein-tyrosine phosphatase family
Alternative Names
CDI1KAP1; CDK2-associated dual specificity phosphatase; CDK2-associated dual-specificity phosphatase; Cdk-associated protein phosphatase; CIP2; cyclin-dependent kinase inhibitor 3; cyclin-dependent kinase interacting protein 2; Cyclin-dependent kinase interactor 1; Cyclin-dependent kinase-interacting protein 2; EC 3.1.3.16; EC 3.1.3.48; FLJ25787; KAPMGC70625; Kinase-associated phosphatase CDKN3 CDI1, CIP2, KAP, KAP1 cyclin dependent kinase inhibitor 3 cyclin-dependent kinase inhibitor 3|CDK2-associated dual specificity phosphatase|Cdk-associated protein phosphatase|cyclin-dependent kinase inhibitor|cyclin-dependent kinase interacting protein 2|cyclin-dependent kinase interactor 1|kinase-associated phosphatase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CDKN3, check out the CDKN3 Infographic
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In this infographic, you will see the following information for CDKN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CDKN3 (NM_005192) Human Over-expression Lysate (LS001033)
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No publications found for LS001033
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