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Product Info Summary
| SKU: | A05157 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CDKN3 Antibody
SKU/Catalog Number
A05157
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CDKN3 Antibody catalog # A05157. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDKN3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05157)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CDKN3. AA range:31-80
Reactive Species
A05157 is reactive to CDKN3 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
23.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05157 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of SH-SY5Y cells using CDKN3 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from LOVO cells, using CDKN3 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-CDKN3 Antibody (A05157)
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Customer Q&As
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3 Customer Q&As for Anti-CDKN3 Antibody
Question
Is a blocking peptide available for product anti-CDKN3 antibody (A05157)?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
We do provide the blocking peptide for product anti-CDKN3 antibody (A05157). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-17
Question
We are currently using anti-CDKN3 antibody A05157 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The anti-CDKN3 antibody (A05157) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-14
Question
Do you have a BSA free version of anti-CDKN3 antibody A05157 available?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CDKN3 antibody A05157 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-09
