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Product Info Summary
|Description:||Transient overexpression lysate of intraflagellar transport 74 homolog (Chlamydomonas) (IFT74), transcript variant 2|
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CMG1 (IFT74) (NM_001099222) Human Over-expression Lysate
Transient overexpression lysate of intraflagellar transport 74 homolog (Chlamydomonas) (IFT74), transcript variant 2
Storage & Handling
Cite This Product
CMG1 (IFT74) (NM_001099222) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080173)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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CMG1 (IFT74) Human Over-expression Lysates NM_001099222.
Western blot validation of overexpression lysate (LS080173) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IFT74 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for IFT74 (Source: Uniprot.org, NCBI)
Intraflagellar transport protein 74 homolog
Capillary morphogenesis gene 1 protein; CCDC2capillary morphogenesis protein 1; CMG1Coiled-coil domain-containing protein 2; CMG-1MGC111562; coiled-coil domain containing 2; FLJ22621; intraflagellar transport 74 homolog (Chlamydomonas); intraflagellar transport protein 74 homolog IFT74 CCDC2, CMG-1, CMG1 intraflagellar transport 74 intraflagellar transport protein 74 homolog|capillary morphogenesis gene 1 protein|capillary morphogenesis protein 1|coiled-coil domain containing 2|coiled-coil domain-containing protein 2|intraflagellar transport 74 homolog*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on IFT74, check out the IFT74 Infographic
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In this infographic, you will see the following information for IFT74: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for LS080173
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