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Product Info Summary
| SKU: | LS003981 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ligase IV, DNA, ATP-dependent (LIG4), transcript variant 1 |
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Product info
Product Name
DNA Ligase IV (LIG4) (NM_002312) Human Over-expression Lysate
See all DNA Ligase IV products
SKU/Catalog Number
LS003981
Size
100 µg
Description
Transient overexpression lysate of ligase IV, DNA, ATP-dependent (LIG4), transcript variant 1
Storage & Handling
Cite This Product
DNA Ligase IV (LIG4) (NM_002312) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003981)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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DNA Ligase IV (LIG4) Human Over-expression Lysates NM_001098268.
Western blot validation of overexpression lysate (LS003981) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with LIG4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
DNA Ligase IV (LIG4) Human Over-expression Lysates NM_002312.
Western blot validation of overexpression lysate (LS003981) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with LIG4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
DNA Ligase IV (LIG4) Human Over-expression Lysates NM_206937.
Western blot validation of overexpression lysate (LS003981) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with LIG4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for LIG4 (Source: Uniprot.org, NCBI)
Gene Name
LIG4
Full Name
DNA ligase 4
Weight
103.971kDa
Superfamily
ATP-dependent DNA ligase family
Alternative Names
DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; EC 6.5.1.1; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase LIG4 LIG4S DNA ligase 4 DNA ligase 4|DNA joinase|DNA ligase IV|DNA repair enzyme|ligase IV, DNA, ATP-dependent|polydeoxyribonucleotide synthase [ATP] 4|polynucleotide ligase|sealase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on LIG4, check out the LIG4 Infographic
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In this infographic, you will see the following information for LIG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DNA Ligase IV (LIG4) (NM_002312) Human Over-expression Lysate (LS003981)
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