Anti-DNA Ligase IV Rabbit Monoclonal Antibody

Boster Bio Anti-DNA Ligase IV Rabbit Monoclonal Antibody catalog # M01685. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M01685
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-DNA Ligase IV Rabbit Monoclonal Antibody

See all DNA Ligase IV products

SKU/Catalog Number

M01685

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DNA Ligase IV Rabbit Monoclonal Antibody catalog # M01685. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DNA Ligase IV Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01685)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADHG-12

Immunogen

A synthesized peptide derived from human DNA Ligase IV

Reactive Species

M01685 is reactive to LIG4 in Human

Applications

M01685 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500~1:2000
IHC 1:50~1:200
ICC/IF 1:50~1:200

Validation Images & Assay Conditions

Gene/Protein Information For LIG4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P49917

Gene ID

3981

Gene Name

LIG4

Full Name

DNA ligase 4

Weight

103.971kDa

Superfamily

ATP-dependent DNA ligase family

Alternative Names

DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; EC 6.5.1.1; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase LIG4 LIG4S DNA ligase 4 DNA ligase 4|DNA joinase|DNA ligase IV|DNA repair enzyme|ligase IV, DNA, ATP-dependent|polydeoxyribonucleotide synthase [ATP] 4|polynucleotide ligase|sealase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LIG4, check out the LIG4 Infographic

LIG4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LIG4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-DNA Ligase IV Rabbit Monoclonal Antibody

Question

Do you have a BSA free version of anti-DNA Ligase IV Monoclonal antibody M01685 available?

W. Williams

Verified customer

Asked: 2020-05-05

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DNA Ligase IV Monoclonal antibody M01685 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-05-05

Question

Please see the WB image, lot number and protocol we used for bone marrow using anti-DNA Ligase IV Monoclonal antibody M01685. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-29

Question

I have a question about product M01685, anti-DNA Ligase IV Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01685 anti-DNA Ligase IV Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-03

Question

I see that the anti-DNA Ligase IV Monoclonal antibody M01685 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-10-25

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-10-25

Question

Is this M01685 anti-DNA Ligase IV Monoclonal antibody reactive to the isotypes of LIG4?

T. Huang

Verified customer

Asked: 2017-10-31

Answer

The immunogen of M01685 anti-DNA Ligase IV Monoclonal antibody is A synthesized peptide derived from human DNA Ligase IV Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D) J recombination. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-10-31

Question

I was wanting to use your anti-DNA Ligase IV Monoclonal antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?

L. Parker

Verified customer

Asked: 2017-04-07

Answer

As indicated on the product datasheet, M01685 anti-DNA Ligase IV Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-04-07

Question

We are currently using anti-DNA Ligase IV Monoclonal antibody M01685 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

V. Mitchell

Verified customer

Asked: 2013-09-05

Answer

The anti-DNA Ligase IV Monoclonal antibody (M01685) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-09-05

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M01685
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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