Click image to see more details
Product Info Summary
| SKU: | M01685 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-DNA Ligase IV Rabbit Monoclonal Antibody
SKU/Catalog Number
M01685
BM5459 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DNA Ligase IV Rabbit Monoclonal Antibody catalog # M01685. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA Ligase IV Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01685)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADHG-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DNA Ligase IV
Reactive Species
M01685 is reactive to LIG4 in Human
Observed Molecular Weight
103 kDa
Calculated molecular weight
104.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01685 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of DNA Ligase IV expression in Ramos cell lysate.
Click image to see more details
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
Specific Publications For Anti-DNA Ligase IV Rabbit Monoclonal Antibody (M01685)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-DNA Ligase IV Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-DNA Ligase IV Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-DNA Ligase IV Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-DNA Ligase IV Monoclonal antibody M01685 available?
W. Williams
Verified customer
Asked: 2020-05-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DNA Ligase IV Monoclonal antibody M01685 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-05
Question
Please see the WB image, lot number and protocol we used for bone marrow using anti-DNA Ligase IV Monoclonal antibody M01685. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-29
Question
I have a question about product M01685, anti-DNA Ligase IV Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01685 anti-DNA Ligase IV Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-03
Question
I see that the anti-DNA Ligase IV Monoclonal antibody M01685 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-10-25
Question
Is this M01685 anti-DNA Ligase IV Monoclonal antibody reactive to the isotypes of LIG4?
T. Huang
Verified customer
Asked: 2017-10-31
Answer
The immunogen of M01685 anti-DNA Ligase IV Monoclonal antibody is A synthesized peptide derived from human DNA Ligase IV Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D) J recombination. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-31
Question
I was wanting to use your anti-DNA Ligase IV Monoclonal antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
L. Parker
Verified customer
Asked: 2017-04-07
Answer
As indicated on the product datasheet, M01685 anti-DNA Ligase IV Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-07
Question
We are currently using anti-DNA Ligase IV Monoclonal antibody M01685 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
V. Mitchell
Verified customer
Asked: 2013-09-05
Answer
The anti-DNA Ligase IV Monoclonal antibody (M01685) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-05
