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- Table of Contents
DNA ligase 4 Antibodies
AND LIG4 ELISA kits, DNA ligase 4 Proteins
Many biological assays use antibodies to detect DNA ligase 4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DNA ligase 4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DNA ligase 4 antibodies...
Designing a Western blot for DNA ligase 4? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the LIG4 Western Blot GuideLIG4 Infographic by Boster Bio
All LIG4 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, Flow Cytometry, IF, ICC, WB
$370
Cat # A01685-3
100 µg/vial
Cat # A01685-3
100 µg/vial
LIG4 Overview
Facts about DNA ligase 4.
DNA ligase 4 (LIG4)
Efficiently joins single-strand fractures in a double- stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.
The LIG4-XRCC4 complex accounts for the NHEJ ligation step, and XRCC4 enhances the linking activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is determined by the assembly of the DNA- dependent protein kinase complex DNA-PK to these DNA ends.
Gene Information
| Human | |
|---|---|
| Gene Name: | LIG4 |
| Uniprot: | P49917 |
| Entrez: | 3981 |
Family
| Belongs to: |
|---|
| ATP-dependent DNA ligase family |
Alternative Names
DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; EC 6.5.1.1; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase
Molecular Weight
Mass (kDA):
103.971 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 13q33.3 |
| Sequence: | 13; NC_000013.11 (108207442..108218349, complement) |
Tissue Specificity
Testis, thymus, prostate and heart.
Subcellular Localizations
Nucleus.
Diseases
- Malignant Neoplasms
- Neoplasms
- Immunologic Deficiency Syndromes
- Genomic Instability
- Chromosomal Translocation
- Malignant Neoplasm Of Breast
- Congenital Combined Immunodeficiency
- Severe Combined Immunodeficiency
- Mammary Neoplasms
- Lymphoma
- Microcephaly
- Developmental Delay (disorder)
- Ataxia
Interacting Proteins
- APLF
- DCLRE1C
- NHEJ1
- XRCC4
Pathways
- Dna Repair
- V(d)j Recombination
- Double-strand Break Repair
- Dna Replication
- Cell Cycle
- Hypersensitivity
- Cell Cycle Checkpoint
- Sporulation
- Senescence
- Cell Growth
- Cell Proliferation
- Mismatch Repair
- Regeneration
PTMs
- Phosphorylation
Research Areas
- Breast Cancer
- Cancer
- DNA Double Strand Break Repair
- DNA Repair
- Non-homologous end-joining
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/LIG4
bosterbio.com/bosterbio-gene-info-cards/LIG4
References
PMID: 7760816 by Wei Y.-F., et al. Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination.
PMID: 8798671 by Robins P., et al. DNA ligase IV from HeLa cell nuclei.